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| | <StructureSection load='6q4s' size='340' side='right'caption='[[6q4s]], [[Resolution|resolution]] 1.83Å' scene=''> | | <StructureSection load='6q4s' size='340' side='right'caption='[[6q4s]], [[Resolution|resolution]] 1.83Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6q4s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_chartreusis_nrrl_3882 Streptomyces chartreusis nrrl 3882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q4S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q4S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6q4s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_chartreusis_NRRL_3882 Streptomyces chartreusis NRRL 3882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Q4S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">penA, SCNRRL3882_7461 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1079985 Streptomyces chartreusis NRRL 3882])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pentalenene_synthase Pentalenene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.7 4.2.3.7] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6q4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q4s OCA], [https://pdbe.org/6q4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6q4s RCSB], [https://www.ebi.ac.uk/pdbsum/6q4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6q4s ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q4s OCA], [http://pdbe.org/6q4s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q4s RCSB], [http://www.ebi.ac.uk/pdbsum/6q4s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q4s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A2N9BKY0_STRCX A0A2N9BKY0_STRCX] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pentalenene synthase]]
| + | [[Category: Streptomyces chartreusis NRRL 3882]] |
| - | [[Category: Streptomyces chartreusis nrrl 3882]] | + | [[Category: Correia Cordeiro RS]] |
| - | [[Category: Cordeiro, R S.Correia]] | + | [[Category: Hakansson M]] |
| - | [[Category: Hakansson, M]] | + | [[Category: Kourist R]] |
| - | [[Category: Kourist, R]] | + | [[Category: Logan DT]] |
| - | [[Category: Logan, D T]] | + | |
| - | [[Category: Plant protein]]
| + | |
| - | [[Category: Sesquiterpene]]
| + | |
| - | [[Category: Terpene synthase]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
A0A2N9BKY0_STRCX
Publication Abstract from PubMed
With more than 50,000 members, terpenoids are one of the most important classes of natural products and show an enormous diversity. Due to their unique odors and specific bioactivities they already find wide application in the flavor, fragrance and pharma industries. Since most terpenoids can only be obtained by natural product extraction, the discovery of biosynthetic genes for the generation of terpene diversity becomes increasingly important. This study describes the discovery of three novel sesquiterpene synthases from Streptomyces chartreusis with preference for the formation of germacradiene-11-ol, alpha-eudesmol and alpha-amorphene respectively. The alpha-eudesmol synthase showed formation of 10-epi-delta-eudesmol and elemol as side products. Eudesmol-isomers are known to have repellent activity, which makes this enzyme a potential catalyst for products for the prevention of mosquito-related disease. The determination of the structure of the apo-enzyme of alpha-eudesmol synthase from S. chartreusis provides the first structural insights into an eudesmol-forming enzyme.
Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an alpha-eudesmol synthase.,Kracht ON, Correia Cordeiro RS, Hakansson M, Stockmann J, Sander D, Bandow J, Senges CHR, Logan DT, Kourist R J Biotechnol. 2019 Mar 27. pii: S0168-1656(19)30079-3. doi:, 10.1016/j.jbiotec.2019.03.006. PMID:30928538[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kracht ON, Correia Cordeiro RS, Hakansson M, Stockmann J, Sander D, Bandow J, Senges CHR, Logan DT, Kourist R. Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an alpha-eudesmol synthase. J Biotechnol. 2019 Mar 27. pii: S0168-1656(19)30079-3. doi:, 10.1016/j.jbiotec.2019.03.006. PMID:30928538 doi:http://dx.doi.org/10.1016/j.jbiotec.2019.03.006
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