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| | <StructureSection load='6scd' size='340' side='right'caption='[[6scd]], [[Resolution|resolution]] 1.35Å' scene=''> | | <StructureSection load='6scd' size='340' side='right'caption='[[6scd]], [[Resolution|resolution]] 1.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6scd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ccug_64165 Ccug 64165]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SCD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SCD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6scd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halopseudomonas_aestusnigri Halopseudomonas aestusnigri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SCD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6sbn|6sbn]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B7O88_11480 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=857252 CCUG 64165])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6scd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6scd OCA], [https://pdbe.org/6scd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6scd RCSB], [https://www.ebi.ac.uk/pdbsum/6scd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6scd ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6scd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6scd OCA], [http://pdbe.org/6scd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6scd RCSB], [http://www.ebi.ac.uk/pdbsum/6scd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6scd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A1H6AD45_9GAMM A0A1H6AD45_9GAMM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ccug 64165]] | + | [[Category: Halopseudomonas aestusnigri]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bollinger, A]] | + | [[Category: Bollinger A]] |
| - | [[Category: Hoeppner, A]] | + | [[Category: Hoeppner A]] |
| - | [[Category: Jaeger, K E]] | + | [[Category: Jaeger K-E]] |
| - | [[Category: Kobus, S]] | + | [[Category: Kobus S]] |
| - | [[Category: Smits, S H.J]] | + | [[Category: Smits SHJ]] |
| - | [[Category: Thies, S]] | + | [[Category: Thies S]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Marine bacteria]]
| + | |
| - | [[Category: Pet hydrolase]]
| + | |
| - | [[Category: Polyester degradation]]
| + | |
| - | [[Category: Pseudomonas aestusnigri]]
| + | |
| Structural highlights
6scd is a 2 chain structure with sequence from Halopseudomonas aestusnigri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.35Å |
| Ligands: | , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
A0A1H6AD45_9GAMM
Publication Abstract from PubMed
Biodegradation of synthetic polymers, in particular polyethylene terephthalate (PET), is of great importance, since environmental pollution with PET and other plastics has become a severe global problem. Here, we report on the polyester degrading ability of a novel carboxylic ester hydrolase identified in the genome of the marine hydrocarbonoclastic bacterium Pseudomonas aestusnigri VGXO14 (T) . The enzyme, designated PE-H, belongs to the type IIa family of PET hydrolytic enzymes as indicated by amino acid sequence homology. It was produced in Escherichia coli, purified and its crystal structure was solved at 1.09 A resolution representing the first structure of a type IIa PET hydrolytic enzyme. The structure shows a typical alpha/beta-hydrolase fold and high structural homology to known polyester hydrolases. PET hydrolysis was detected at 30 degrees C with amorphous PET film (PETa), but not with PET film from a commercial PET bottle (PETb). A rational mutagenesis study to improve the PET degrading potential of PE-H yielded variant PE-H (Y250S) which showed improved activity, ultimately also allowing the hydrolysis of PETb. The crystal structure of this variant solved at 1.35 A resolution allowed to rationalize the improvement of enzymatic activity. A PET oligomer binding model was proposed by molecular docking computations. Our results indicate a significant potential of the marine bacterium P. aestusnigri for PET degradation.
A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri - Structural and Functional Insights.,Bollinger A, Thies S, Knieps-Grunhagen E, Gertzen C, Kobus S, Hoppner A, Ferrer M, Gohlke H, Smits SHJ, Jaeger KE Front Microbiol. 2020 Feb 13;11:114. doi: 10.3389/fmicb.2020.00114. eCollection, 2020. PMID:32117139[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bollinger A, Thies S, Knieps-Grunhagen E, Gertzen C, Kobus S, Hoppner A, Ferrer M, Gohlke H, Smits SHJ, Jaeger KE. A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri - Structural and Functional Insights. Front Microbiol. 2020 Feb 13;11:114. doi: 10.3389/fmicb.2020.00114. eCollection, 2020. PMID:32117139 doi:http://dx.doi.org/10.3389/fmicb.2020.00114
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