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| | <StructureSection load='6sjj' size='340' side='right'caption='[[6sjj]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='6sjj' size='340' side='right'caption='[[6sjj]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6sjj]] is a 36 chain structure with sequence from [http://en.wikipedia.org/wiki/Hyppe Hyppe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SJJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sjj]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Hypericum_perforatum Hypericum perforatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SJJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AN:8-ANILINO-1-NAPHTHALENE+SULFONATE'>2AN</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PR10.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65561 HYPPE])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AN:8-ANILINO-1-NAPHTHALENE+SULFONATE'>2AN</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sjj OCA], [http://pdbe.org/6sjj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sjj RCSB], [http://www.ebi.ac.uk/pdbsum/6sjj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sjj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sjj OCA], [https://pdbe.org/6sjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sjj RCSB], [https://www.ebi.ac.uk/pdbsum/6sjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sjj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A160HRF0_HYPPE A0A160HRF0_HYPPE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hyppe]] | + | [[Category: Hypericum perforatum]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dauter, Z]] | + | [[Category: Dauter Z]] |
| - | [[Category: Gilski, M]] | + | [[Category: Gilski M]] |
| - | [[Category: Jaskolski, M]] | + | [[Category: Jaskolski M]] |
| - | [[Category: Sliwiak, J]] | + | [[Category: Sliwiak J]] |
| - | [[Category: Smietanska, J]] | + | [[Category: Smietanska J]] |
| - | [[Category: Strzalka, R]] | + | [[Category: Strzalka R]] |
| - | [[Category: Wolny, J]] | + | [[Category: Wolny J]] |
| - | [[Category: Commensurately modulated superstructure]]
| + | |
| - | [[Category: Cytokinin]]
| + | |
| - | [[Category: Depression]]
| + | |
| - | [[Category: Glycine-rich loop]]
| + | |
| - | [[Category: Hydrophobic cavity]]
| + | |
| - | [[Category: Hypericin]]
| + | |
| - | [[Category: Pathogenesis-related protein]]
| + | |
| - | [[Category: Phytohormone binding]]
| + | |
| - | [[Category: Plant defense]]
| + | |
| - | [[Category: Plant hormone binding]]
| + | |
| - | [[Category: Plant protein]]
| + | |
| - | [[Category: Pr-10 fold]]
| + | |
| - | [[Category: Pr-10 protein]]
| + | |
| - | [[Category: Tetartohedral twinning]]
| + | |
| Structural highlights
6sjj is a 36 chain structure with sequence from Hypericum perforatum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.3Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
A0A160HRF0_HYPPE
Publication Abstract from PubMed
Superstructure modulation, with violation of the strict short-range periodic order of consecutive crystal unit cells, is well known in small-molecule crystallography but is rarely reported for macromolecular crystals. To date, one modulated macromolecular crystal structure has been successfully determined and refined for a pathogenesis-related class 10 protein from Hypericum perforatum (Hyp-1) crystallized as a complex with 8-anilinonaphthalene-1-sulfonate (ANS) [Sliwiak et al. (2015), Acta Cryst. D71, 829-843]. The commensurate modulation in that case was interpreted in a supercell with sevenfold expansion along c. When crystallized in the additional presence of melatonin, the Hyp-1-ANS complex formed crystals with a different pattern of structure modulation, in which the supercell shows a ninefold expansion of c, manifested in the diffraction pattern by a wave of reflection-intensity modulation with crests at l = 9n and l = 9n +/- 4. Despite complicated tetartohedral twinning, the structure has been successfully determined and refined to 2.3 A resolution using a description in a ninefold-expanded supercell, with 36 independent Hyp-1 chains and 156 ANS ligands populating the three internal (95 ligands) and five interstitial (61 ligands) binding sites. The commensurate superstructures and ligand-binding sites of the two crystal structures are compared, with a discussion of the effect of melatonin on the co-crystallization process.
A new modulated crystal structure of the ANS complex of the St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell.,Smietanska J, Sliwiak J, Gilski M, Dauter Z, Strzalka R, Wolny J, Jaskolski M Acta Crystallogr D Struct Biol. 2020 Jul 1;76(Pt 7):653-667. doi:, 10.1107/S2059798320006841. Epub 2020 Jun 17. PMID:32627738[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Smietanska J, Sliwiak J, Gilski M, Dauter Z, Strzalka R, Wolny J, Jaskolski M. A new modulated crystal structure of the ANS complex of the St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell. Acta Crystallogr D Struct Biol. 2020 Jul 1;76(Pt 7):653-667. doi:, 10.1107/S2059798320006841. Epub 2020 Jun 17. PMID:32627738 doi:http://dx.doi.org/10.1107/S2059798320006841
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