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6syf

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Current revision (12:50, 24 January 2024) (edit) (undo)
 
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====
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==Human Ubc9 with covalent isopeptide ligand==
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<StructureSection load='6syf' size='340' side='right'caption='[[6syf]]' scene=''>
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<StructureSection load='6syf' size='340' side='right'caption='[[6syf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6syf]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SYF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SYF FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6syf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syf OCA], [http://pdbe.org/6syf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6syf RCSB], [http://www.ebi.ac.uk/pdbsum/6syf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6syf ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6syf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syf OCA], [https://pdbe.org/6syf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6syf RCSB], [https://www.ebi.ac.uk/pdbsum/6syf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6syf ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Enzymes are powerful tools for protein labelling due to their specificity and mild reaction conditions. Many protocols, however, are restricted to modifications at protein termini, rely on non-peptidic metabolites or require large recognition domains. Here we report a chemoenzymatic method, which we call lysine acylation using conjugating enzymes (LACE), to site-specifically modify folded proteins at internal lysine residues. LACE relies on a minimal genetically encoded tag (four residues) recognized by the E2 small ubiquitin-like modifier-conjugating enzyme Ubc9, and peptide or protein thioesters. Together, this approach obviates the need for E1 and E3 enzymes, enabling isopeptide formation with just Ubc9 in a programmable manner. We demonstrate the utility of LACE by the site-specific attachment of biochemical probes, one-pot dual-labelling in combination with sortase, and the conjugation of wild-type ubiquitin and ISG15 to recombinant proteins.
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Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins.,Hofmann R, Akimoto G, Wucherpfennig TG, Zeymer C, Bode JW Nat Chem. 2020 Sep 14. pii: 10.1038/s41557-020-0528-y. doi:, 10.1038/s41557-020-0528-y. PMID:32929246<ref>PMID:32929246</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6syf" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[SUMO conjugating enzyme Ubc9|SUMO conjugating enzyme Ubc9]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Z-disk]]
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[[Category: Akimoto G]]
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[[Category: Bode JW]]
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[[Category: Hofmann R]]
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[[Category: Wucherpfennig TG]]
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[[Category: Zeymer C]]

Current revision

Human Ubc9 with covalent isopeptide ligand

PDB ID 6syf

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