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6yhd

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(New page: ==tRNA-guanine Transglycosylase (TGT) labeled with 5-fluorotryptophan in co-crystallized complex with 6-amino-4-(2-((2R,3S,4R,5R)-3,4-dihydroxy-5-methoxytetrahydrofuran-2-yl)ethyl)-2-(met...)
Current revision (13:27, 24 January 2024) (edit) (undo)
 
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<StructureSection load='6yhd' size='340' side='right'caption='[[6yhd]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
<StructureSection load='6yhd' size='340' side='right'caption='[[6yhd]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6yhd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YHD FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6yhd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zymomonas_mobilis_subsp._mobilis_ZM4_=_ATCC_31821 Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YHD FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1WK:METHYL+6-[6-AMINO-2-(METHYLAMINO)-8-OXO-7,8-DIHYDRO-1H-IMIDAZO[4,5-G]QUINAZOLIN-4-YL]-5,6-DIDEOXY-BETA-D-RIBO-HEXOFURANOSIDE'>1WK</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FTR:FLUOROTRYPTOPHANE'>FTR</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1WK:METHYL+6-[6-AMINO-2-(METHYLAMINO)-8-OXO-7,8-DIHYDRO-1H-IMIDAZO[4,5-G]QUINAZOLIN-4-YL]-5,6-DIDEOXY-BETA-D-RIBO-HEXOFURANOSIDE'>1WK</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FTR:FLUOROTRYPTOPHANE'>FTR</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6yh2|6yh2]], [[6ygz|6ygz]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhd OCA], [https://pdbe.org/6yhd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yhd RCSB], [https://www.ebi.ac.uk/pdbsum/6yhd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhd ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-guanine(34)_transglycosylase tRNA-guanine(34) transglycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhd OCA], [http://pdbe.org/6yhd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yhd RCSB], [http://www.ebi.ac.uk/pdbsum/6yhd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhd ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]
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[https://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]
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==See Also==
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*[[TRNA-guanine transglycosylase 3D structures|TRNA-guanine transglycosylase 3D structures]]
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Heine, A]]
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[[Category: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821]]
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[[Category: Klebe, G]]
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[[Category: Heine A]]
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[[Category: Nguyen, A]]
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[[Category: Klebe G]]
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[[Category: 19f nmr]]
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[[Category: Nguyen A]]
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[[Category: Guanine insertion enzyme]]
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[[Category: Homodimer]]
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[[Category: Tgt]]
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[[Category: Transferase]]
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[[Category: Trna-guanine transglycosylase]]
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Current revision

tRNA-guanine Transglycosylase (TGT) labeled with 5-fluorotryptophan in co-crystallized complex with 6-amino-4-(2-((2R,3S,4R,5R)-3,4-dihydroxy-5-methoxytetrahydrofuran-2-yl)ethyl)-2-(methylamino)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one

PDB ID 6yhd

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