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8u6x
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==ATP-dependent DNA ligase Lig E from Neisseria gonorrhoeae== | |
| + | <StructureSection load='8u6x' size='340' side='right'caption='[[8u6x]], [[Resolution|resolution]] 2.44Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8u6x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae_MS11 Neisseria gonorrhoeae MS11] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U6X FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.44Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u6x OCA], [https://pdbe.org/8u6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u6x RCSB], [https://www.ebi.ac.uk/pdbsum/8u6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u6x ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D6HB39_NEIGO D6HB39_NEIGO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | BACKGROUND: The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisseria gonorrhoeae and characterised its growth and infection phenotype. RESULTS: This demonstrates that N. gonorrhoeae Lig E is a non-essential gene and its deletion does not cause defects in replication or survival of DNA-damaging stressors. Knock-out strains were partially defective in biofilm formation on an artificial surface as well as adhesion to epithelial cells. In addition to in vivo characterisation, we have recombinantly expressed and assayed N. gonorrhoeae Lig E and determined the crystal structure of the enzyme-adenylate engaged with DNA substrate in an open non-catalytic conformation. CONCLUSIONS: These findings, coupled with the predicted extracellular/ periplasmic location of Lig E indicates a role in extracellular DNA joining as well as providing insight into the binding dynamics of these minimal DNA ligases. | ||
| - | + | A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation.,Pan J, Singh A, Hanning K, Hicks J, Williamson A BMC Microbiol. 2024 Jan 20;24(1):29. doi: 10.1186/s12866-024-03193-9. PMID:38245708<ref>PMID:38245708</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8u6x" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Neisseria gonorrhoeae MS11]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Pan J]] | ||
| + | [[Category: Williamson A]] | ||
Current revision
ATP-dependent DNA ligase Lig E from Neisseria gonorrhoeae
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