7avr
From Proteopedia
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==The tetrameric structure of haloalkane dehalogenase DpaA from Paraglaciecola agarilytica NO2== | ==The tetrameric structure of haloalkane dehalogenase DpaA from Paraglaciecola agarilytica NO2== | ||
| - | <StructureSection load='7avr' size='340' side='right'caption='[[7avr]]' scene=''> | + | <StructureSection load='7avr' size='340' side='right'caption='[[7avr]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AVR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7avr]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Paraglaciecola_agarilytica_NO2 Paraglaciecola agarilytica NO2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AVR FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7avr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7avr OCA], [https://pdbe.org/7avr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7avr RCSB], [https://www.ebi.ac.uk/pdbsum/7avr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7avr ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7avr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7avr OCA], [https://pdbe.org/7avr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7avr RCSB], [https://www.ebi.ac.uk/pdbsum/7avr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7avr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/K6XNL5_9ALTE K6XNL5_9ALTE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Haloalkane dehalogenases (EC 3.8.1.5) are microbial enzymes that catalyse the hydrolytic conversion of halogenated compounds, resulting in a halide ion, a proton and an alcohol. These enzymes are used in industrial biocatalysis, bioremediation and biosensing of environmental pollutants or for molecular tagging in cell biology. The novel haloalkane dehalogenase DpaA described here was isolated from the psychrophilic and halophilic bacterium Paraglaciecola agarilytica NO2, which was found in marine sediment collected from the East Sea near Korea. Gel-filtration experiments and size-exclusion chromatography provided information about the dimeric composition of the enzyme in solution. The DpaA enzyme was crystallized using the sitting-drop vapour-diffusion method, yielding rod-like crystals that diffracted X-rays to 2.0 A resolution. Diffraction data analysis revealed a case of merohedral twinning, and subsequent structure modelling and refinement resulted in a tetrameric model of DpaA, highlighting an uncommon multimeric nature for a protein belonging to haloalkane dehalogenase subfamily I. | ||
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| + | The tetrameric structure of the novel haloalkane dehalogenase DpaA from Paraglaciecola agarilytica NO2.,Mazur A, Prudnikova T, Grinkevich P, Mesters JR, Mrazova D, Chaloupkova R, Damborsky J, Kuty M, Kolenko P, Kuta Smatanova I Acta Crystallogr D Struct Biol. 2021 Mar 1;77(Pt 3):347-356. doi:, 10.1107/S2059798321000486. Epub 2021 Feb 17. PMID:33645538<ref>PMID:33645538</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7avr" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Dehalogenase 3D structures|Dehalogenase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Paraglaciecola agarilytica NO2]] | ||
[[Category: Grinkevich P]] | [[Category: Grinkevich P]] | ||
[[Category: Kolenko P]] | [[Category: Kolenko P]] | ||
Current revision
The tetrameric structure of haloalkane dehalogenase DpaA from Paraglaciecola agarilytica NO2
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