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7ax2
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7ax2 is ON HOLD Authors: Mylemans, B., Vandebroek, L., Parac-Vogt, T.N., Voet, A.R.D. Description: Crystal structure of the computationally designe...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the computationally designed Scone-E protein co-crystallized with STA, form b== | |
| + | <StructureSection load='7ax2' size='340' side='right'caption='[[7ax2]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7ax2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AX2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=S5T:Monolacunary+Keggin+(STA)'>S5T</scene>, <scene name='pdbligand=SIW:Keggin+(STA)'>SIW</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ax2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ax2 OCA], [https://pdbe.org/7ax2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ax2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ax2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ax2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Recent years have seen an increase in the development of computational proteins, including symmetric ones. A ninefold-symmetric beta-propeller protein named Cake has recently been developed. Here, attempts were made to further engineer this protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon the addition of a polyoxometalate, highlighting the usefulness of these molecules as crystallization additives. | ||
| - | + | Crystal structures of Scone: pseudosymmetric folding of a symmetric designer protein.,Mylemans B, Killian T, Vandebroek L, Van Meervelt L, Tame JRH, Parac-Vogt TN, Voet ARD Acta Crystallogr D Struct Biol. 2021 Jul 1;77(Pt 7):933-942. doi:, 10.1107/S2059798321005787. Epub 2021 Jun 18. PMID:34196619<ref>PMID:34196619</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Mylemans | + | <div class="pdbe-citations 7ax2" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Vandebroek | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Mylemans B]] | ||
| + | [[Category: Parac-Vogt TN]] | ||
| + | [[Category: Vandebroek L]] | ||
| + | [[Category: Voet ARD]] | ||
Current revision
Crystal structure of the computationally designed Scone-E protein co-crystallized with STA, form b
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