This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7bnp
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7bnp is ON HOLD until sometime in the future Authors: Trinh, N.T., Cambillau, C., Leone, P. Description: Llama nanobody nb17 raised against GldL fr...) |
|||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Llama nanobody nb17 raised against GldL from Flavobacterium jonhsoniae== | |
| + | <StructureSection load='7bnp' size='340' side='right'caption='[[7bnp]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7bnp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BNP FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bnp OCA], [https://pdbe.org/7bnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bnp RCSB], [https://www.ebi.ac.uk/pdbsum/7bnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bnp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | GldL is an inner-membrane protein that is essential for the function of the type IX secretion system (T9SS) in Flavobacterium johnsoniae. The complex that it forms with GldM is supposed to act as a new rotary motor involved in the gliding motility of the bacterium. In the context of structural studies of GldL to gain information on the assembly and function of the T9SS, two camelid nanobodies were selected, produced and purified. Their interaction with the cytoplasmic domain of GldL was characterized and their crystal structures were solved. These nanobodies will be used as crystallization chaperones to help in the crystallization of the cytoplasmic domain of GldL and could also help to solve the structure of the complex using molecular replacement. | ||
| - | + | Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae.,Trinh TTN, Gaubert A, Melani P, Cambillau C, Roussel A, Leone P Acta Crystallogr F Struct Biol Commun. 2021 Jun 1;77(Pt 6):171-176. doi:, 10.1107/S2053230X21005185. Epub 2021 May 24. PMID:34100775<ref>PMID:34100775</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Cambillau | + | <div class="pdbe-citations 7bnp" style="background-color:#fffaf0;"></div> |
| - | [[Category: Leone | + | |
| - | [[Category: Trinh | + | ==See Also== |
| + | *[[Antibody 3D structures|Antibody 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Lama glama]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cambillau C]] | ||
| + | [[Category: Leone P]] | ||
| + | [[Category: Trinh NT]] | ||
Current revision
Llama nanobody nb17 raised against GldL from Flavobacterium jonhsoniae
| |||||||||||
