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7zsc
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7zsc is ON HOLD Authors: Lebedev, A., Koski, M.K., Wierenga, R.K., Murthy, A.V., Sulu, R. Description: Crystal structure of the heterodimeric human...) |
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| - | '''Unreleased structure''' | ||
| - | The entry | + | ==Crystal structure of the heterodimeric human C-P4H-II with truncated alpha subunit (C-P4H-II delta281)== |
| + | <StructureSection load='7zsc' size='340' side='right'caption='[[7zsc]], [[Resolution|resolution]] 3.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7zsc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZSC FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.85Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zsc OCA], [https://pdbe.org/7zsc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zsc RCSB], [https://www.ebi.ac.uk/pdbsum/7zsc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zsc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Disease == | ||
| + | [https://www.uniprot.org/uniprot/P4HA2_HUMAN P4HA2_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/P4HA2_HUMAN P4HA2_HUMAN] Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Collagen prolyl 4-hydroxylases (C-P4H) are alpha2beta2 tetramers, which catalyze the prolyl 4-hydroxylation of procollagen chains, allowing for the formation of the stable triple-helical collagen structure in the endoplasmic reticulum. The C-P4H alpha-subunit provides the N-terminal dimerization domain, the middle peptide-substrate-binding domain (PSB), and the C-terminal catalytic (CAT) domain, while the beta-subunit is identical to the enzyme protein disulfide isomerase (PDI). The structure of the N-terminal part of the alpha-subunit (N-terminal and PSB domain) is known, but the structures of the PSB-CAT linker region and the CAT domain as well as its mode of assembly with the beta/PDI-subunit, are not known. Here we report the crystal structure of the CAT domain of human C-P4H-II complexed with the intact beta/PDI-subunit, at 3.8A resolution. The CAT domain interacts with the a, b', and a' domains of the beta/PDI-subunit, such that the CAT active site is facing bulk solvent. The structure also shows that the C-P4H-II CAT domain has a unique N-terminal extension, consisting of alpha-helices and a beta-strand, which is the edge strand of its major antiparallel beta-sheet. This extra region of the CAT domain interacts tightly with the beta/PDI-subunit, showing that the CAT-PDI interface includes an inter-subunit disulfide bridge with the a' domain and tight hydrophobic interactions with the b' domain. Using this new structural information, the structure of the mature C-P4H-II alpha2beta2 tetramer is predicted. The model suggests that the CAT active site properties are modulated by alpha-helices of the N-terminal dimerization domains of both subunits of the alpha2-dimer. | ||
| - | + | Crystal structure of the collagen prolyl 4-hydroxylase (C-P4H) catalytic domain complexed with PDI: towards a model of the C-P4H alpha2beta2 tetramer.,Murthy AV, Sulu R, Lebedev A, Salo AM, Korhonen K, Venkatesan R, Tu H, Bergmann U, Janis J, Laitaoja M, Ruddock L, Myllyharju J, Koski MK, Wierenga RK J Biol Chem. 2022 Oct 17:102614. doi: 10.1016/j.jbc.2022.102614. PMID:36265586<ref>PMID:36265586</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Koski | + | <div class="pdbe-citations 7zsc" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Murthy | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Koski MK]] | ||
| + | [[Category: Lebedev A]] | ||
| + | [[Category: Murthy AV]] | ||
| + | [[Category: Sulu R]] | ||
| + | [[Category: Wierenga RK]] | ||
Current revision
Crystal structure of the heterodimeric human C-P4H-II with truncated alpha subunit (C-P4H-II delta281)
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