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| - | [[Image:111d.jpg|left|200px]] | |
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| - | {{Structure
| + | ==CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS== |
| - | |PDB= 111d |SIZE=350|CAPTION= <scene name='initialview01'>111d</scene>, resolution 2.250Å
| + | <StructureSection load='111d' size='340' side='right'caption='[[111d]], [[Resolution|resolution]] 2.25Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[111d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=111D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=111D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=111d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=111d OCA], [https://pdbe.org/111d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=111d RCSB], [https://www.ebi.ac.uk/pdbsum/111d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=111d ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=111d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=111d OCA], [http://www.ebi.ac.uk/pdbsum/111d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=111d RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Booth ED]] |
| - | '''CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS'''
| + | [[Category: Brown T]] |
| - | | + | [[Category: Chambers J]] |
| - | | + | [[Category: Leonard GA]] |
| - | ==Overview==
| + | |
| - | The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis clearly shows that the mismatches are of the form A(anti).G(syn). Thermal denaturation studies indicate that the stability of the duplex is strongly pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is stabilized by protonation. Three different arrangements have been observed for base-pairs between guanine and adenine and it is likely that A.G mismatch conformation is strongly influenced by dipole-dipole interactions with adjacent base-pairs.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 111D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=111D OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2754734 2754734]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Booth, E D.]] | + | |
| - | [[Category: Brown, T.]] | + | |
| - | [[Category: Chambers, J.]] | + | |
| - | [[Category: Leonard, G A.]] | + | |
| - | [[Category: b-dna]]
| + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: mismatched]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:26:45 2008''
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