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168d

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STABILIZING EFFECTS OF THE RNA 2'-SUBSTITUENT: CRYSTAL STRUCTURE OF AN OLIGODEOXYNUCLEOTIDE DUPLEX CONTAINING 2'-O-METHYLATED ADENOSINES

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Retrieved from "http://52.214.119.220/wiki/index.php/168d"

Categories: Large Structures | Egli M | Lubini P | Zuercher W

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-[[Image:168d.jpg|left|200px]]<br /><applet load="168d" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="168d, resolution 2.000&Aring;" />
-'''STABILIZING EFFECTS OF THE RNA 2'-SUBSTITUENT: CRYSTAL STRUCTURE OF AN OLIGODEOXYNUCLEOTIDE DUPLEX CONTAINING ;2'-O-METHYLATED ADENOSINES ;'''<br />
-==Overview==
+==STABILIZING EFFECTS OF THE RNA 2'-SUBSTITUENT: CRYSTAL STRUCTURE OF AN OLIGODEOXYNUCLEOTIDE DUPLEX CONTAINING 2'-O-METHYLATED ADENOSINES==
-BACKGROUND: The stability of hybrids of 2'-O-methyl-ribonucleotides with, complementary RNA is considerably higher than that of the corresponding, DNA.RNA duplexes. The 2'-O-modified ribonucleotides are thus an attractive, class of compounds for antisense applications. Understanding how these, substituents stabilize the structure of the hybrid duplex may be important, in the design of ribonucleotides with novel properties. RESULTS: The, crystal structure of a dimer of the self-complementary DNA strand, d(GCGT)O2'mer(A)d(TACGC), which has a 2'-O-methylated ribonucleotide, incorporated at position 5, was determined at 2.1 A resolution. This, strand forms a duplex with an overall A-type conformation; the methyl, groups of the two modified adenosines point into the relatively wide minor, groove. Both 2'-methoxy groups are hydrogen-bonded to solvent molecules., These results allowed us to build a model of a fully 2'-O-methylated RNA, double helix. CONCLUSIONS: Insertion of 2'-O-modified RNA residues into a, stretch of DNA can nucleate a local A-type conformation, in part because, modification with a bulky residue at this position stabilizes a C3'-endo, type sugar pucker. The increased stability of fully 2'-O-methylated RNA, may result from hydrophobic interactions between substituents in the minor, groove. As the 2'-O-methyl groups are directed into the minor groove, it, may be worthwhile to introduce tailor-made 2'-O-substituents into RNA; it, might be possible to design groups that both stabilize the hybrid duplexes, and carry a nuclease function, further improving the efficacy of these, modified RNAs in antisense applications.
+<StructureSection load='168d' size='340' side='right'caption='[[168d]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[168d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=168D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=168D FirstGlance]. <br>
-D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=168D OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2M:2-O-METHYLADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>A2M</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=168d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=168d OCA], [https://pdbe.org/168d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=168d RCSB], [https://www.ebi.ac.uk/pdbsum/168d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=168d ProSAT]</span></td></tr>
-Stabilizing effects of the RNA 2'-substituent: crystal structure of an oligodeoxynucleotide duplex containing 2'-O-methylated adenosines., Lubini P, Zurcher W, Egli M, Chem Biol. 1994 Sep;1(1):39-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9383369 9383369]
+</table>
-[[Category: Protein complex]]
+__TOC__
-[[Category: Egli, M.]]
+</StructureSection>
-[[Category: Lubini, P.]]
+[[Category: Large Structures]]
-[[Category: Zuercher, W.]]
+[[Category: Egli M]]
-[[Category: a-dna/rna]]
+[[Category: Lubini P]]
-[[Category: double helix]]
+[[Category: Zuercher W]]
-[[Category: modified]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:03:03 2007''

168d

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STABILIZING EFFECTS OF THE RNA 2'-SUBSTITUENT: CRYSTAL STRUCTURE OF AN OLIGODEOXYNUCLEOTIDE DUPLEX CONTAINING 2'-O-METHYLATED ADENOSINES

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