1a12

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[[Image:1a12.gif|left|200px]]<br /><applet load="1a12" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a12, resolution 1.7&Aring;" />
 
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'''REGULATOR OF CHROMOSOME CONDENSATION (RCC1) OF HUMAN'''<br />
 
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==Overview==
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==REGULATOR OF CHROMOSOME CONDENSATION (RCC1) OF HUMAN==
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The gene encoding the regulator of chromosome condensation (RCC1) was cloned by virtue of its ability to complement the temperature-sensitive phenotype of the hamster cell line tsBN2, which undergoes premature chromosome condensation or arrest in the G1 phase of the cell cycle at non-permissive temperatures. RCC1 homologues have been identified in many eukaryotes, including budding and fission yeast. Mutations in the gene affect pre-messenger RNA processing and transport, mating, initiation of mitosis and chromatin decondensation, suggesting that RCC1 is important in the control of nucleo-cytoplasmic transport and the cell cycle. Biochemically, RCC1 is a guanine-nucleotide-exchange factor for the nuclear Ras homologue Ran; it increases the dissociation of Ran-bound GDP by 10(5)-fold. It may also bind to DNAvia a protein-protein complex. Here we show that the structure of human RCC1, solved to 1.7-A resolution by X-ray crystallography, consists of a seven-bladed propeller formed from internal repeats of 51-68 residues per blade. The sequence and structure of the repeats differ from those of WD40-domain proteins, which also form seven-bladed propellers and include the beta-subunits of G proteins. The nature of the structure explains the consequences of a wide range of known mutations. The region of the protein that is involved in guanine-nucleotide exchange is located opposite the region that is thought to be involved in chromosome binding.
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<StructureSection load='1a12' size='340' side='right'caption='[[1a12]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1a12]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A12 FirstGlance]. <br>
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Known disease associated with this structure: Alveolar soft-part sarcoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=606236 606236]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a12 OCA], [https://pdbe.org/1a12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a12 RCSB], [https://www.ebi.ac.uk/pdbsum/1a12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a12 ProSAT]</span></td></tr>
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==About this Structure==
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</table>
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1A12 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A12 OCA].
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== Function ==
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[https://www.uniprot.org/uniprot/RCC1_HUMAN RCC1_HUMAN] Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.<ref>PMID:1944575</ref>
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==Reference==
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== Evolutionary Conservation ==
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The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller., Renault L, Nassar N, Vetter I, Becker J, Klebe C, Roth M, Wittinghofer A, Nature. 1998 Mar 5;392(6671):97-101. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9510255 9510255]
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a1/1a12_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a12 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Becker, J.]]
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[[Category: Becker J]]
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[[Category: Nassar, N.]]
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[[Category: Nassar N]]
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[[Category: Renault, L.]]
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[[Category: Renault L]]
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[[Category: Roth, M.]]
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[[Category: Roth M]]
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[[Category: Vetter, I.]]
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[[Category: Vetter I]]
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[[Category: Wittinghofer, A.]]
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[[Category: Wittinghofer A]]
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[[Category: gef]]
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[[Category: guanine nucleotide exchange factor]]
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[[Category: ran]]
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[[Category: ras-like nuclear gtp binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:40 2008''
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Current revision

REGULATOR OF CHROMOSOME CONDENSATION (RCC1) OF HUMAN

PDB ID 1a12

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