1a6j

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[[Image:1a6j.gif|left|200px]]<br />
 
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<applet load="1a6j" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a6j, resolution 2.35&Aring;" />
 
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'''NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN'''<br />
 
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==Overview==
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==NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN==
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The bacterial rpoN operon codes for sigma 54, which is the key sigma, factor that, under nitrogen starvation conditions, activates the, transcription of genes needed to assimilate ammonia and glutamate. The, rpoN operon contains several other open reading frames that are, cotranscribed with sigma 54. The product of one of these, the 17.9 kDa, protein IIANtr, is homologous to IIA proteins of the, phosphoenolpyruvate:sugar phosphotransferase (PTS) system. IIANtr, influences the transcription of sigma 54-dependent genes through an, unknown mechanism and may thereby provide a regulatory link between carbon, and nitrogen metabolism. Here we describe the 2.35 A X-ray structure of, Escherichia coli IIANtr. It is the first structure of a IIA enzyme from, the fructose-mannitol family of the PTS. ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9636714 (full description)]]
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<StructureSection load='1a6j' size='340' side='right'caption='[[1a6j]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1a6j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A6J FirstGlance]. <br>
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1A6J is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4 and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69]]. Structure known Active Site: . Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A6J OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a6j OCA], [https://pdbe.org/1a6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a6j RCSB], [https://www.ebi.ac.uk/pdbsum/1a6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a6j ProSAT]</span></td></tr>
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The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli., Bordo D, van Monfort RL, Pijning T, Kalk KH, Reizer J, Saier MH Jr, Dijkstra BW, J Mol Biol. 1998 May 29;279(1):245-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9636714 9636714]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PTSN_ECOLI PTSN_ECOLI] Seems to have a role in regulating nitrogen assimilation (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a6j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a6j ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bordo, D.]]
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[[Category: Bordo D]]
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[[Category: Dijkstra, B.W.]]
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[[Category: Dijkstra BW]]
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[[Category: Kalk, K.H.]]
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[[Category: Kalk KH]]
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[[Category: Montfort, R.Van.]]
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[[Category: Pijning T]]
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[[Category: Pijning, T.]]
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[[Category: Reizer J]]
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[[Category: Reizer, J.]]
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[[Category: Saier MH]]
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[[Category: Saier, M.H.]]
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[[Category: Van Montfort R]]
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[[Category: BME]]
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[[Category: SO4]]
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[[Category: nitrogen regulation]]
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[[Category: phosphotransferase system]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:48:31 2007''
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Current revision

NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN

PDB ID 1a6j

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