1a77

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1a77" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a77, resolution 2.00&Aring;" /> '''FLAP ENDONUCLEASE-1 ...)
Current revision (06:28, 7 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1a77.jpg|left|200px]]<br /><applet load="1a77" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1a77, resolution 2.00&Aring;" />
 
-
'''FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII'''<br />
 
-
==Overview==
+
==FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII==
-
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an, essential enzyme for eukaryotic DNA replication and repair. The crystal, structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A, resolution, reveals an active site with two metal ions residing on top of, a deep cleft where several conserved acidic residues are clustered. Near, the active site, a long flexible loop comprised of many basic and aromatic, residues forms a hole large enough to accommodate the DNA substrate., Deletion mutations in this loop significantly decreased the nuclease, activity and specificity of FEN-1, suggesting that the loop is critical, for recognition and cleavage of the junction between single and, double-stranded regions of flap DNA.
+
<StructureSection load='1a77' size='340' side='right'caption='[[1a77]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1a77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A77 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a77 OCA], [https://pdbe.org/1a77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a77 RCSB], [https://www.ebi.ac.uk/pdbsum/1a77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a77 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/FEN_METJA FEN_METJA] Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/1a77_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a77 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1A77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A77 OCA].
+
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii., Hwang KY, Baek K, Kim HY, Cho Y, Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9699635 9699635]
+
[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
-
[[Category: Single protein]]
+
[[Category: Baek K]]
-
[[Category: Baek, K.]]
+
[[Category: Cho Y]]
-
[[Category: Cho, Y.]]
+
[[Category: Hwang KY]]
-
[[Category: Hwang, K.Y.]]
+
[[Category: Kim H]]
-
[[Category: Kim, H.]]
+
-
[[Category: MG]]
+
-
[[Category: 5'-3' exo/endo nuclease]]
+
-
[[Category: dna repair]]
+
-
[[Category: dna replication]]
+
-
[[Category: rad27]]
+
-
[[Category: rth]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:39:28 2007''
+

Current revision

FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII

PDB ID 1a77

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1a77"
Personal tools