1af3

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[[Image:1af3.gif|left|200px]]<br /><applet load="1af3" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1af3, resolution 2.5&Aring;" />
 
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'''RAT BCL-XL AN APOPTOSIS INHIBITORY PROTEIN'''<br />
 
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==Overview==
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==RAT BCL-XL AN APOPTOSIS INHIBITORY PROTEIN==
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Bcl-xL is a member of the Bcl-2 protein family, which regulates apoptosis. Preparation of recombinant rat Bcl-xL yielded two forms, one deamidated at -Asn-Gly- sequences to produce isoaspartates and the other not deamidated. The crystal structures of the two forms show that they both adopt an essentially identical backbone structure which resembles the fold of human Bcl-xL: three layers of two alpha-helices each, capped at one end by two short helices. Both forms have a long disordered region, which contains the potential deamidation sites. The molecular structure exhibits a low level of interhelical interactions, the presence of three cavities, and a notable hydrophobic cleft surrounded by walls rich in basic residues. These unique structural features may be favorable for its accommodation into membranes or for possible rearrangement to modulate homo-/heterodimerization. Homology modeling of Bcl-2 and Bax, based on the Bcl-xL structure, suggests that Bax has the strongest potential for membrane insertion. Furthermore, we found a possible interface for interaction with non-Bcl-2 family member proteins, such as CED-4 homologues.
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<StructureSection load='1af3' size='340' side='right'caption='[[1af3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1af3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AF3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1af3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af3 OCA], [https://pdbe.org/1af3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1af3 RCSB], [https://www.ebi.ac.uk/pdbsum/1af3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1af3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B2CL1_RAT B2CL1_RAT] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:8662675</ref> <ref>PMID:7828536</ref> <ref>PMID:18250306</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/1af3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1af3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AF3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF3 OCA].
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*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of rat Bcl-xL. Implications for the function of the Bcl-2 protein family., Aritomi M, Kunishima N, Inohara N, Ishibashi Y, Ohta S, Morikawa K, J Biol Chem. 1997 Oct 31;272(44):27886-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9346936 9346936]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Aritomi M]]
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[[Category: Aritomi, M.]]
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[[Category: Inohara N]]
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[[Category: Inohara, N.]]
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[[Category: Ishibashi Y]]
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[[Category: Ishibashi, Y.]]
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[[Category: Kunishima N]]
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[[Category: Kunishima, N.]]
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[[Category: Morikawa K]]
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[[Category: Morikawa, K.]]
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[[Category: Ohta S]]
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[[Category: Ohta, S.]]
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[[Category: alternative splicing]]
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[[Category: apoptosis]]
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[[Category: bcl-xl]]
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[[Category: mitochondrion]]
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[[Category: regulatory protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:52 2008''
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Current revision

RAT BCL-XL AN APOPTOSIS INHIBITORY PROTEIN

PDB ID 1af3

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