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1agx

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[[Image:1agx.gif|left|200px]]<br /><applet load="1agx" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1agx, resolution 2.9&Aring;" />
 
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'''REFINED CRYSTAL STRUCTURE OF ACINETOBACTER GLUTAMINASIFICANS GLUTAMINASE-ASPARAGINASE'''<br />
 
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==Overview==
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==REFINED CRYSTAL STRUCTURE OF ACINETOBACTER GLUTAMINASIFICANS GLUTAMINASE-ASPARAGINASE==
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The crystal structure of glutaminase-asparaginase from Acinetobacter glutaminasificans has been reinterpreted and refined to an R factor of 0.171 at 2.9 A resolution, using the same X-ray diffraction data that were used to build a preliminary model of this enzyme [Ammon, Weber, Wlodawer, Harrison, Gilliland, Murphy, Sjolin &amp; Roberts (1988). J. Biol. Chem. 263, 150-156]. The current model, which does not include solvent, is based in part on the related structure of Escherichia coli asparaginase and is significantly different from the structure of the enzyme from A. glutaminasificans described previously. The reason for the discrepancies has been traced to insufficient phasing power of the original heavy-atom derivative data, which could not be compensated for fully by electron-density modification techniques. The corrected structure of A. glutaminasificans glutaminase-asparaginase is presented and compared with the preliminary model and with the structure of E. coli asparaginase.
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<StructureSection load='1agx' size='340' side='right'caption='[[1agx]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1agx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_glutaminasificans Acinetobacter glutaminasificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AGX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1agx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1agx OCA], [https://pdbe.org/1agx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1agx RCSB], [https://www.ebi.ac.uk/pdbsum/1agx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1agx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ASPQ_ACIGL ASPQ_ACIGL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ag/1agx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1agx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AGX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acinetobacter_glutaminasificans Acinetobacter glutaminasificans]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGX OCA].
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*[[Glutaminase 3D structures|Glutaminase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Refined crystal structure of Acinetobacter glutaminasificans glutaminase-asparaginase., Lubkowski J, Wlodawer A, Housset D, Weber IT, Ammon HL, Murphy KC, Swain AL, Acta Crystallogr D Biol Crystallogr. 1994 Nov 1;50(Pt 6):826-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299349 15299349]
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[[Category: Acinetobacter glutaminasificans]]
[[Category: Acinetobacter glutaminasificans]]
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[[Category: Asparaginase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Ammon HL]]
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[[Category: Ammon, H L.]]
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[[Category: Housset D]]
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[[Category: Housset, D.]]
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[[Category: Lubkowski J]]
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[[Category: Lubkowski, J.]]
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[[Category: Murphy KC]]
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[[Category: Murphy, K C.]]
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[[Category: Swain AL]]
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[[Category: Swain, A L.]]
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[[Category: Weber IT]]
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[[Category: Weber, I T.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: bacterial amidohydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:44:21 2008''
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Current revision

REFINED CRYSTAL STRUCTURE OF ACINETOBACTER GLUTAMINASIFICANS GLUTAMINASE-ASPARAGINASE

PDB ID 1agx

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