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1ajn

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[[Image:1ajn.gif|left|200px]]<br />
 
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<applet load="1ajn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ajn, resolution 2.36&Aring;" />
 
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'''PENICILLIN ACYLASE COMPLEXED WITH P-NITROPHENYLACETIC ACID'''<br />
 
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==Overview==
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==PENICILLIN ACYLASE COMPLEXED WITH P-NITROPHENYLACETIC ACID==
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The enzyme penicillin acylase (penicillin amidohydrolase EC 3.5.1. 11), catalyses the cleavage of the amide bond in the benzylpenicillin, (penicillin G) side-chain to produce phenylacetic acid and, 6-aminopenicillanic acid (6-APA). The enzyme is of great pharmaceutical, importance, as the product 6-APA is the starting point for the synthesis, of many semi-synthetic penicillin antibiotics. Studies have shown that the, enzyme is specific for hydrolysis of phenylacetamide derivatives, but is, more tolerant of features in the rest of the substrate. It is this, property that has led to many other applications for the enzyme, and, greater knowledge of the enzyme's structure and specificity could, facilitate engineering of the enzyme, enhancing its potential for chemical, and industrial ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9813130 (full description)]]
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<StructureSection load='1ajn' size='340' side='right'caption='[[1ajn]], [[Resolution|resolution]] 2.36&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ajn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AJN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.36&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AAN:2-(4-NITROPHENYL)ACETIC+ACID'>AAN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ajn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ajn OCA], [https://pdbe.org/1ajn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ajn RCSB], [https://www.ebi.ac.uk/pdbsum/1ajn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ajn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PAC_ECOLX PAC_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aj/1ajn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ajn ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AJN is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with CA and AAN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]]. Structure known Active Sites: CAA and CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AJN OCA]].
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*[[Penicillin acylase|Penicillin acylase]]
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__TOC__
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==Reference==
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</StructureSection>
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Ligand-induced conformational change in penicillin acylase., Done SH, Brannigan JA, Moody PC, Hubbard RE, J Mol Biol. 1998 Nov 27;284(2):463-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9813130 9813130]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Penicillin amidase]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Done SH]]
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[[Category: Done, S.H.]]
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[[Category: AAN]]
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[[Category: CA]]
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[[Category: antibiotic resistance]]
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[[Category: hydrolase]]
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[[Category: ligand induced conformational change]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:48:56 2007''
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Current revision

PENICILLIN ACYLASE COMPLEXED WITH P-NITROPHENYLACETIC ACID

PDB ID 1ajn

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