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1ajz

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[[Image:1ajz.gif|left|200px]]
 
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==STRUCTURE OF DIHYDROPTEROATE PYROPHOSPHORYLASE==
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The line below this paragraph, containing "STRUCTURE_1ajz", creates the "Structure Box" on the page.
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<StructureSection load='1ajz' size='340' side='right'caption='[[1ajz]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ajz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AJZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1ajz| PDB=1ajz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ajz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ajz OCA], [https://pdbe.org/1ajz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ajz RCSB], [https://www.ebi.ac.uk/pdbsum/1ajz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ajz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHPS_ECOLI DHPS_ECOLI] DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.<ref>PMID:368012</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aj/1ajz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ajz ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF DIHYDROPTEROATE PYROPHOSPHORYLASE'''
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==See Also==
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*[[Dihydropteroate synthase 3D structures|Dihydropteroate synthase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Sulfonamides were amongst the first clinically useful antibacterial agents to be discovered. The identification of sulfanilamide as the active component of the dye Prontosil rubrum led to the synthesis of clinically useful analogues. Today sulfamethoxazole (in combination with trimethoprim), is used to treat urinary tract infections caused by bacteria such as Escherichia coli and is also a first-line treatment for pneumonia caused by the fungus Pneumocystis carinii, a common condition in AIDS patients. The site of action is the de novo folate biosynthesis enzyme dihydropteroate synthase (DHPS) where sulfonamides act as analogues of one of the substrates, para-aminobenzoic acid (pABA). We report here the crystal structure of E.coli DHPS at 2.0 A resolution refined to an R-factor of 0.185. The single domain of 282 residues forms an eight-stranded alpha/beta-barrel. The 7,8-dihydropterin pyrophosphate (DHPPP) substrate binds in a deep cleft in the barrel, whilst sulfanilamide binds closer to the surface. The DHPPP ligand site is highly conserved amongst prokaryotic and eukaryotic DHPSs.
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__TOC__
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</StructureSection>
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==About this Structure==
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1AJZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJZ OCA].
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==Reference==
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Crystal structure of the anti-bacterial sulfonamide drug target dihydropteroate synthase., Achari A, Somers DO, Champness JN, Bryant PK, Rosemond J, Stammers DK, Nat Struct Biol. 1997 Jun;4(6):490-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9187658 9187658]
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[[Category: Dihydropteroate synthase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Achari, A.]]
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[[Category: Achari A]]
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[[Category: Bryant, P K.]]
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[[Category: Bryant PK]]
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[[Category: Champness, J N.]]
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[[Category: Champness JN]]
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[[Category: Rosemond, J.]]
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[[Category: Rosemond J]]
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[[Category: Somers, D O.]]
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[[Category: Somers DO]]
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[[Category: Stammers, D K.]]
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[[Category: Stammers DK]]
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[[Category: Antibiotic]]
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[[Category: Biosynthesis]]
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[[Category: Folate]]
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[[Category: Resistance]]
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[[Category: Synthase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:22:07 2008''
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Current revision

STRUCTURE OF DIHYDROPTEROATE PYROPHOSPHORYLASE

PDB ID 1ajz

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