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1alw

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(New page: 200px<br /><applet load="1alw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1alw, resolution 2.03&Aring;" /> '''INHIBITOR AND CALCIU...)
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[[Image:1alw.gif|left|200px]]<br /><applet load="1alw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1alw, resolution 2.03&Aring;" />
 
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'''INHIBITOR AND CALCIUM BOUND DOMAIN VI OF PORCINE CALPAIN'''<br />
 
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==Overview==
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==INHIBITOR AND CALCIUM BOUND DOMAIN VI OF PORCINE CALPAIN==
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The three dimensional structure of calcium-bound domain VI of porcine, calpain has been determined to 1.9 A resolution. The crystal structure, reveals five EF-hands, one more than previously suggested. There are two, EF-hand pairs, one pair (EF1-EF2) displays an 'open' conformation and the, other (EF3-EF4) a 'closed' conformation. Unusually, a calcium atom is, found at the C-terminal end of the calcium binding loop of EF4. With two, additional residues in the calcium binding loop, the fifth EF-hand (EF5), is in a 'closed' conformation. EF5 pairs up with the corresponding fifth, EF-hand of a non-crystallographically related molecule. Considering the, EF5's role in a homodimer formation of domain VI, we suggest a model for, the assembly of heterodimeric calpain. The crystal structure of a Ca2+, bound domain VI-inhibitor (PD150606) complex has been refined to 2.1 A, resolution. A possible mode for calpain inhibition is discussed.
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<StructureSection load='1alw' size='340' side='right'caption='[[1alw]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1alw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ALW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ALW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ISA:3-(4-IODO-PHENYL)-2-MERCAPTO-PROPIONIC+ACID'>ISA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1alw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1alw OCA], [https://pdbe.org/1alw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1alw RCSB], [https://www.ebi.ac.uk/pdbsum/1alw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1alw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPNS1_PIG CPNS1_PIG] Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/al/1alw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1alw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ALW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with CA and ISA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.52 and 3.4.22.53 3.4.22.52 and 3.4.22.53] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ALW OCA].
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*[[Calpain 3D structures|Calpain 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of calcium bound domain VI of calpain at 1.9 A resolution and its role in enzyme assembly, regulation, and inhibitor binding., Lin GD, Chattopadhyay D, Maki M, Wang KK, Carson M, Jin L, Yuen PW, Takano E, Hatanaka M, DeLucas LJ, Narayana SV, Nat Struct Biol. 1997 Jul;4(7):539-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9228946 9228946]
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[[Category: Large Structures]]
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[[Category: Hydrolase]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Lin, G.]]
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[[Category: Lin G]]
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[[Category: Narayana, S.V.L.]]
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[[Category: Narayana SVL]]
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[[Category: CA]]
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[[Category: ISA]]
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[[Category: calcium binding]]
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[[Category: calmodulin like]]
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[[Category: domain of cystein protease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:57:14 2007''
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Current revision

INHIBITOR AND CALCIUM BOUND DOMAIN VI OF PORCINE CALPAIN

PDB ID 1alw

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