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1an7

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[[Image:1an7.gif|left|200px]]
 
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{{Structure
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==RIBOSOMAL PROTEIN S8 FROM THERMUS THERMOPHILUS==
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|PDB= 1an7 |SIZE=350|CAPTION= <scene name='initialview01'>1an7</scene>, resolution 2.9&Aring;
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<StructureSection load='1an7' size='340' side='right'caption='[[1an7]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1an7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AN7 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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|GENE= RPS8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1an7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1an7 OCA], [https://pdbe.org/1an7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1an7 RCSB], [https://www.ebi.ac.uk/pdbsum/1an7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1an7 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1an7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1an7 OCA], [http://www.ebi.ac.uk/pdbsum/1an7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1an7 RCSB]</span>
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[https://www.uniprot.org/uniprot/RS8_THETH RS8_THETH] One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit (By similarity).
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/an/1an7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1an7 ConSurf].
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<div style="clear:both"></div>
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'''RIBOSOMAL PROTEIN S8 FROM THERMUS THERMOPHILUS'''
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==See Also==
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*[[Ribosomal protein S8|Ribosomal protein S8]]
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__TOC__
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==Overview==
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</StructureSection>
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S8 is one of the core ribosomal proteins. It binds to 16 S RNA with high affinity and independently of other ribosomal proteins. It also acts as a translational repressor in Escherichia coli by binding to its own mRNA. The structure of Thermus thermophilus S8 has been determined by the method of multiple isomorphous replacement at 2.9 A resolution and refined to a crystallographic R-factor of 16.2% (Rfree 27.5%). The two domains of the structure have an alpha/beta fold and are connected by a long protruding loop. The two molecules in the asymmetric unit of the crystal interact through an extensive hydrophobic core and form a tightly associated dimer, while symmetry-related molecules form a joint beta-sheet of mixed type. This type of protein-protein interaction could be realized within the ribosomal assembly. A comparison of the structures of T. thermophilus and Bacillus stearothermophilus S8 shows that the interdomain loop is eight residues longer in the former and reveals high structural conservation of an extensive region, located in the C-terminal domain. From mutational studies this region was proposed earlier to be involved in specific interaction with RNA. On the basis of these data and on the comparison of the two structures of S8, it is proposed that the three-dimensional structure of specific RNA binding sites in ribosomal proteins is highly conserved among different species.
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[[Category: Large Structures]]
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==About this Structure==
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1AN7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AN7 OCA].
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==Reference==
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Crystal structure of ribosomal protein S8 from Thermus thermophilus reveals a high degree of structural conservation of a specific RNA binding site., Nevskaya N, Tishchenko S, Nikulin A, al-Karadaghi S, Liljas A, Ehresmann B, Ehresmann C, Garber M, Nikonov S, J Mol Biol. 1998 May 29;279(1):233-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9636713 9636713]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Al-Karadaghi, S.]]
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[[Category: Al-Karadaghi S]]
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[[Category: Nevskaya, N.]]
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[[Category: Nevskaya N]]
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[[Category: Nikonov, S.]]
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[[Category: Nikonov S]]
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[[Category: protein-protein binding]]
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[[Category: ribosomal protein]]
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[[Category: rrna-protein binding]]
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[[Category: thermus thermophilus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:43:52 2008''
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Current revision

RIBOSOMAL PROTEIN S8 FROM THERMUS THERMOPHILUS

PDB ID 1an7

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