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1ayl

From Proteopedia

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[[Image:1ayl.gif|left|200px]]<br /><applet load="1ayl" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ayl, resolution 1.8&Aring;" />
 
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'''PHOSPHOENOLPYRUVATE CARBOXYKINASE'''<br />
 
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==Overview==
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==PHOSPHOENOLPYRUVATE CARBOXYKINASE==
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We report the 1.8 A crystal structure of adenosine triphosphate, (ATP)-magnesium-oxalate bound phosphoenolpyruvate carboxykinase (PCK) from, Escherichia coli. ATP binding induces a 20 degree hinge-like rotation of, the N- and C-terminal domains which closes the active-site cleft. PCK, possesses a novel nucleotide-binding fold, particularly in the, adenine-binding region, where the formation of a cis backbone torsion, angle in a loop glycine residue promotes intimate contacts between the, adenine-binding loop and adenine, while stabilizing a syn conformation of, the base. This complex represents a reaction intermediate analogue along, the pathway of the conversion of oxaloacetate to phosphoenolpyruvate, and, provides insight into the mechanistic details of the chemical reaction, catalysed by this enzyme.
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<StructureSection load='1ayl' size='340' side='right'caption='[[1ayl]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ayl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AYL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ayl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayl OCA], [https://pdbe.org/1ayl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ayl RCSB], [https://www.ebi.ac.uk/pdbsum/1ayl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ayl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PCKA_ECOLI PCKA_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/1ayl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ayl ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AYL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=OXL:'>OXL</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(ATP) Phosphoenolpyruvate carboxykinase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.49 4.1.1.49] Known structural/functional Site: <scene name='pdbsite=ACT:Putative+Active+Site+Residues'>ACT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYL OCA].
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*[[Phosphoenolpyruvate carboxykinase 3D structures|Phosphoenolpyruvate carboxykinase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Snapshot of an enzyme reaction intermediate in the structure of the ATP-Mg2+-oxalate ternary complex of Escherichia coli PEP carboxykinase., Tari LW, Matte A, Pugazhenthi U, Goldie H, Delbaere LT, Nat Struct Biol. 1996 Apr;3(4):355-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8599762 8599762]
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[[Category: Escherichia coli K-12]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Phosphoenolpyruvate carboxykinase (ATP)]]
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[[Category: Delbaere LTJ]]
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[[Category: Single protein]]
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[[Category: Goldie H]]
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[[Category: Delbaere, L.T.J.]]
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[[Category: Pugazenthi U]]
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[[Category: Goldie, H.]]
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[[Category: Tari LW]]
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[[Category: Pugazenthi, U.]]
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[[Category: Tari, L.W.]]
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[[Category: ATP]]
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[[Category: MG]]
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[[Category: OXL]]
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[[Category: kinase (transphosphorylating)]]
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[[Category: nucleotide-triphosphate hydrolase]]
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[[Category: p-loop]]
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[[Category: protein-atp complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:32:07 2008''
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Current revision

PHOSPHOENOLPYRUVATE CARBOXYKINASE

PDB ID 1ayl

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