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1baw

From Proteopedia

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[[Image:1baw.gif|left|200px]]<br /><applet load="1baw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1baw, resolution 2.8&Aring;" />
 
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'''PLASTOCYANIN FROM PHORMIDIUM LAMINOSUM'''<br />
 
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==Overview==
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==PLASTOCYANIN FROM PHORMIDIUM LAMINOSUM==
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The crystal structure of the 'blue' copper protein plastocyanin from the, cyanobacterium Phormidium laminosum has been solved and refined using 2.8, A X--ray data. P. laminosum plastocyanin crystallizes in space group, P43212 with unit-cell dimensions a = 86.57, c = 91.47 A and with three, protein molecules per asymmetric unit. The final residual R is 19.9%. The, structure was solved using molecular replacement with a search model based, on the crystal structure of a close homologue, Anabaena variabilis, plastocyanin (66% sequence identity). The molecule of P. laminosum, plastocyanin has 105 amino-acid residues. The single Cu atom is, coordinated by the same residues - two histidines, a cysteine and a, methionine - as in other plastocyanins. In the crystal structure, the, three molecules of the asymmetric unit are related by a, non-crystallographic threefold axis. A Zn atom lies between each pair of, neighbouring molecules in this ensemble, being coordinated by a surface, histidine residue of one molecule and by two aspartates of the other.
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<StructureSection load='1baw' size='340' side='right'caption='[[1baw]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1baw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Phormidium_laminosum Phormidium laminosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BAW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1baw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1baw OCA], [https://pdbe.org/1baw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1baw RCSB], [https://www.ebi.ac.uk/pdbsum/1baw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1baw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLAS_PHOLA PLAS_PHOLA] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ba/1baw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1baw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phormidium_laminosum Phormidium laminosum] with <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=CUA:Cu+Binding+Site'>CUA</scene>, <scene name='pdbsite=CUB:Cu+Binding+Site'>CUB</scene> and <scene name='pdbsite=CUC:Cu+Binding+Site'>CUC</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BAW OCA].
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*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The structure of plastocyanin from the cyanobacterium Phormidium laminosum., Bond CS, Bendall DS, Freeman HC, Guss JM, Howe CJ, Wagner MJ, Wilce MC, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):414-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089349 10089349]
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[[Category: Large Structures]]
[[Category: Phormidium laminosum]]
[[Category: Phormidium laminosum]]
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[[Category: Single protein]]
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[[Category: Bendall DS]]
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[[Category: Bendall, D.S.]]
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[[Category: Bond CS]]
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[[Category: Bond, C.S.]]
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[[Category: Freeman HC]]
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[[Category: Freeman, H.C.]]
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[[Category: Guss JM]]
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[[Category: Guss, J.M.]]
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[[Category: Howe CJ]]
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[[Category: Howe, C.J.]]
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[[Category: Wagner MJ]]
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[[Category: Wagner, M.J.]]
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[[Category: CU]]
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[[Category: ZN]]
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[[Category: blue copper protein]]
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[[Category: cyanobacteria]]
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[[Category: electron transfer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:32:29 2008''
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Current revision

PLASTOCYANIN FROM PHORMIDIUM LAMINOSUM

PDB ID 1baw

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