1bdf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1bdf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bdf, resolution 2.5&Aring;" /> '''STRUCTURE OF ESCHERIC...)
Current revision (06:35, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1bdf.gif|left|200px]]<br /><applet load="1bdf" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1bdf, resolution 2.5&Aring;" />
 
-
'''STRUCTURE OF ESCHERICHIA COLI RNA POLYMERASE ALPHA SUBUNIT N-TERMINAL DOMAIN'''<br />
 
-
==Overview==
+
==STRUCTURE OF ESCHERICHIA COLI RNA POLYMERASE ALPHA SUBUNIT N-TERMINAL DOMAIN==
-
The 2.5 angstrom resolution x-ray crystal structure of the Escherichia, coli RNA polymerase (RNAP) alpha subunit amino-terminal domain (alphaNTD), which is necessary and sufficient to dimerize and assemble the other RNAP, subunits into a transcriptionally active enzyme and contains all of the, sequence elements conserved among eukaryotic alpha homologs, has been, determined. The alphaNTD monomer comprises two distinct, flexibly linked, domains, only one of which participates in the dimer interface. In the, alphaNTD dimer, a pair of helices from one monomer interact with the, cognate helices of the other to form an extensive hydrophobic core. All of, the determinants for interactions with the other RNAP subunits lie on one, face of the alphaNTD dimer. Sequence alignments, combined with, secondary-structure predictions, support proposals that a heterodimer of, the eukaryotic RNAP subunits related to Saccharomyces cerevisiae Rpb3 and, Rpb11 plays the role of the alphaNTD dimer in prokaryotic RNAP.
+
<StructureSection load='1bdf' size='340' side='right'caption='[[1bdf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1bdf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BDF FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bdf OCA], [https://pdbe.org/1bdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bdf RCSB], [https://www.ebi.ac.uk/pdbsum/1bdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bdf ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RPOA_ECOLI RPOA_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bd/1bdf_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bdf ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1BDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BDF OCA].
+
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain., Zhang G, Darst SA, Science. 1998 Jul 10;281(5374):262-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9657722 9657722]
+
-
[[Category: DNA-directed RNA polymerase]]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Darst, S.A.]]
+
[[Category: Darst SA]]
-
[[Category: Zhang, G.]]
+
[[Category: Zhang G]]
-
[[Category: alpha]]
+
-
[[Category: assemble]]
+
-
[[Category: nucleotidyltransferase]]
+
-
[[Category: rnap]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:33:52 2007''
+

Current revision

STRUCTURE OF ESCHERICHIA COLI RNA POLYMERASE ALPHA SUBUNIT N-TERMINAL DOMAIN

PDB ID 1bdf

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bdf"
Personal tools