|
|
| (15 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1bfr.gif|left|200px]] | |
| | | | |
| - | {{Structure
| + | ==IRON STORAGE AND ELECTRON TRANSPORT== |
| - | |PDB= 1bfr |SIZE=350|CAPTION= <scene name='initialview01'>1bfr</scene>, resolution 2.94Å
| + | <StructureSection load='1bfr' size='340' side='right'caption='[[1bfr]], [[Resolution|resolution]] 2.94Å' scene=''> |
| - | |SITE= <scene name='pdbsite=MA:Binuclear+Metal-Binding+Site'>MA</scene>, <scene name='pdbsite=MB:Binuclear+Metal-Binding+Site'>MB</scene>, <scene name='pdbsite=MC:Binuclear+Metal-Binding+Site'>MC</scene>, <scene name='pdbsite=MD:Binuclear+Metal-Binding+Site'>MD</scene>, <scene name='pdbsite=ME:Binuclear+Metal-Binding+Site'>ME</scene>, <scene name='pdbsite=MF:Binuclear+Metal-Binding+Site'>MF</scene>, <scene name='pdbsite=MG:Binuclear+Metal-Binding+Site'>MG</scene>, <scene name='pdbsite=MH:Binuclear+Metal-Binding+Site'>MH</scene>, <scene name='pdbsite=MI:Binuclear+Metal-Binding+Site'>MI</scene>, <scene name='pdbsite=MJ:Binuclear+Metal-Binding+Site'>MJ</scene>, <scene name='pdbsite=MK:Binuclear+Metal-Binding+Site'>MK</scene>, <scene name='pdbsite=ML:Binuclear+Metal-Binding+Site'>ML</scene>, <scene name='pdbsite=MM:Binuclear+Metal-Binding+Site'>MM</scene>, <scene name='pdbsite=MN:Binuclear+Metal-Binding+Site'>MN</scene>, <scene name='pdbsite=MO:Binuclear+Metal-Binding+Site'>MO</scene>, <scene name='pdbsite=MP:Binuclear+Metal-Binding+Site'>MP</scene>, <scene name='pdbsite=MQ:Binuclear+Metal-Binding+Site'>MQ</scene>, <scene name='pdbsite=MR:Binuclear+Metal-Binding+Site'>MR</scene>, <scene name='pdbsite=MS:Binuclear+Metal-Binding+Site'>MS</scene>, <scene name='pdbsite=MT:Binuclear+Metal-Binding+Site'>MT</scene>, <scene name='pdbsite=MU:Binuclear+Metal-Binding+Site'>MU</scene>, <scene name='pdbsite=MV:Binuclear+Metal-Binding+Site'>MV</scene>, <scene name='pdbsite=MW:Binuclear+Metal-Binding+Site'>MW</scene> and <scene name='pdbsite=MX:Binuclear+Metal-Binding+Site'>MX</scene>
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
| + | <table><tr><td colspan='2'>[[1bfr]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BFR FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.94Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bfr OCA], [https://pdbe.org/1bfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bfr RCSB], [https://www.ebi.ac.uk/pdbsum/1bfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bfr ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bfr OCA], [http://www.ebi.ac.uk/pdbsum/1bfr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bfr RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref> |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/1bfr_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bfr ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''IRON STORAGE AND ELECTRON TRANSPORT'''
| + | ==See Also== |
| - | | + | *[[Ferritin 3D structures|Ferritin 3D structures]] |
| - | | + | == References == |
| - | ==Overview== | + | <references/> |
| - | Crystals of E. coli cytochrome b1, alias bacterioferritin, were grown fr om a low ionic strength solution. The resulting monoclniic P21 structure was solved by molecular replacement and refined using noncrystallographi c symmetries applied to the fundamental unit, consisting of two protein subunits and a single haem. From the Patterson self-rotation results it was shown that the asymmetric unit of the monoclinic crystal consists of 12 such dimers and corresponds to a complete, nearly spherical, molecule of bacterioferritin (M4 = 450 kDa) of 432 point-group symmetry. It is thus the most symmetrical cytochrome. As previously determined for the tetragonal form, the haem is located in a special position on a local twofold axis of the dimer. A bimetal centre is also observed within the four-helix bundle of each monomer; a metal-binding site is located on the fourfold axis.
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | ==About this Structure==
| + | |
| - | 1BFR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BFR OCA].
| + | |
| - | | + | |
| - | ==Reference== | + | |
| - | Structure of a monoclinic crystal from of cyctochrome b1 (Bacterioferritin) from E. coli., Dautant A, Meyer JB, Yariv J, Precigoux G, Sweet RM, Kalb AJ, Frolow F, Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):16-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9867433 9867433]
| + | |
| | [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Dautant, A.]] | + | [[Category: Dautant A]] |
| - | [[Category: Frolow, F.]] | + | [[Category: Frolow F]] |
| - | [[Category: Gilboa, A J.Kalb.]]
| + | [[Category: Meyer JB]] |
| - | [[Category: Meyer, J B.]] | + | [[Category: Precigoux G]] |
| - | [[Category: Precigoux, G.]] | + | [[Category: Sweet RM]] |
| - | [[Category: Sweet, R M.]] | + | [[Category: Yariv J]] |
| - | [[Category: Yariv, J.]] | + | |
| - | [[Category: electron transport]]
| + | |
| - | [[Category: iron storage]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:00:01 2008''
| + | |
