1bja

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:36, 7 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1bja.jpg|left|200px]]
 
-
{{Structure
+
==ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA==
-
|PDB= 1bja |SIZE=350|CAPTION= <scene name='initialview01'>1bja</scene>, resolution 2.19&Aring;
+
<StructureSection load='1bja' size='340' side='right'caption='[[1bja]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+
<table><tr><td colspan='2'>[[1bja]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BJA FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
-
|GENE= MOTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T4])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bja OCA], [https://pdbe.org/1bja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bja RCSB], [https://www.ebi.ac.uk/pdbsum/1bja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bja ProSAT]</span></td></tr>
-
 
+
</table>
-
'''ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/MOTA_BPT4 MOTA_BPT4] Required for the transcriptional activation of middle promoters. Middle promoters are characterized by the presence of the conserved sequence [AT]3TGCTTNA (MotA box). MotA binds directly to MotA boxes.
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Bacteriophage T4 encodes a transcription factor, MotA, that binds to the -30 region of middle-mode promoters and activates transcription by host RNA polymerase. We have solved the structure of the MotA activation domain to 2.2 A by X-ray crystallography, and have also determined its secondary structure by NMR. An area on the surface of the protein has a distinctive patch that is populated with acidic and hydrophobic residues. Mutations within this patch cause a defective T4 growth phenotype, arguing that the patch is important for MotA function. One of the mutant MotA activation domains was purified and analyzed by NMR, and the spectra clearly show that the domain is properly folded. The mutant full-length protein appears to bind DNA normally but is deficient in transcriptional activation. We conclude that the acidic/hydrophobic surface patch is specifically involved in transcriptional activation, which is reminiscent of eukaryotic acidic activation domains.
+
[[Category: Escherichia virus T4]]
-
 
+
[[Category: Large Structures]]
-
==About this Structure==
+
[[Category: Cicero MP]]
-
1BJA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJA OCA].
+
[[Category: Davies C]]
-
 
+
[[Category: Finnin MS]]
-
==Reference==
+
[[Category: Kreuzer KN]]
-
The activation domain of the MotA transcription factor from bacteriophage T4., Finnin MS, Cicero MP, Davies C, Porter SJ, White SW, Kreuzer KN, EMBO J. 1997 Apr 15;16(8):1992-2003. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9155025 9155025]
+
[[Category: Porter SJ]]
-
[[Category: Bacteriophage t4]]
+
[[Category: White SW]]
-
[[Category: Single protein]]
+
-
[[Category: Cicero, M P.]]
+
-
[[Category: Davies, C.]]
+
-
[[Category: Finnin, M S.]]
+
-
[[Category: Kreuzer, K N.]]
+
-
[[Category: Porter, S J.]]
+
-
[[Category: White, S W.]]
+
-
[[Category: SO4]]
+
-
[[Category: activation domain]]
+
-
[[Category: alpha helical structure]]
+
-
[[Category: middle mode transcription]]
+
-
[[Category: phage t4]]
+
-
[[Category: transcription regulation]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:11:46 2008''
+

Current revision

ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA

PDB ID 1bja

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bja"
Personal tools