1bow

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(New page: 200px<br /><applet load="1bow" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bow, resolution 2.7&Aring;" /> '''MULTIDRUG-BINDING DOM...)
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[[Image:1bow.jpg|left|200px]]<br /><applet load="1bow" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bow, resolution 2.7&Aring;" />
 
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'''MULTIDRUG-BINDING DOMAIN OF TRANSCRIPTION ACTIVATOR BMRR (APO FORM)'''<br />
 
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==Overview==
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==MULTIDRUG-BINDING DOMAIN OF TRANSCRIPTION ACTIVATOR BMRR (APO FORM)==
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Multidrug-efflux transporters demonstrate an unusual ability to recognize, multiple structurally dissimilar toxins. A comparable ability to bind, diverse hydrophobic cationic drugs is characteristic of the Bacillus, subtilis transcription regulator BmrR, which upon drug binding activates, expression of the multidrug transporter Bmr. Crystal structures of the, multidrug-binding domain of BmrR (2.7 A resolution) and of its complex, with the drug tetraphenylphosphonium (2.8 A resolution) revealed a, drug-induced unfolding and relocation of an alpha helix, which exposes an, internal drug-binding pocket. Tetraphenylphosphonium binding is mediated, by stacking and van der Waals contacts with multiple hydrophobic residues, of the pocket and by an electrostatic interaction between the positively, charged drug and a buried glutamate residue, which is the key to cation, selectivity. Similar binding principles may be used by other, multidrug-binding proteins.
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<StructureSection load='1bow' size='340' side='right'caption='[[1bow]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bow]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOW FirstGlance]. <br>
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1BOW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BOW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bow OCA], [https://pdbe.org/1bow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bow RCSB], [https://www.ebi.ac.uk/pdbsum/1bow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bow ProSAT]</span></td></tr>
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Structural basis of multidrug recognition by BmrR, a transcription activator of a multidrug transporter., Zheleznova EE, Markham PN, Neyfakh AA, Brennan RG, Cell. 1999 Feb 5;96(3):353-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10025401 10025401]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BMRR_BACSU BMRR_BACSU] Activates transcription of the bmr gene in response to structurally dissimilar drugs. Binds rhodamine as an inducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bow_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bow ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brennan, R.G.]]
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[[Category: Brennan RG]]
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[[Category: Markham, P.N.]]
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[[Category: Markham PN]]
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[[Category: Neyfakh, A.A.]]
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[[Category: Neyfakh AA]]
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[[Category: Zheleznova, E.E.]]
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[[Category: Zheleznova EE]]
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[[Category: MN]]
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[[Category: multidrug binding]]
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[[Category: transcription activator]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:48:13 2007''
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Current revision

MULTIDRUG-BINDING DOMAIN OF TRANSCRIPTION ACTIVATOR BMRR (APO FORM)

PDB ID 1bow

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