1bpl

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[[Image:1bpl.gif|left|200px]]<br />
 
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<applet load="1bpl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bpl, resolution 2.2&Aring;" />
 
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'''GLYCOSYLTRANSFERASE'''<br />
 
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==Overview==
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==GLYCOSYLTRANSFERASE==
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The three-dimensional structure of the calcium-free form of Bacillus, licheniformis alpha-amylase (BLA) has been determined by multiple, isomorphous replacement in a crystal of space group P4(3)2(1)2 (a = b =, 119.6 A, c = 85.4 A). The structure was refined using restrained, crystallographic refinement to an R-factor of 0.177 for 28,147 independent, reflections with intensities FObs &gt; 0 at 2.2 A resolution, with root mean, square deviations of 0.008 A and 1.4 degrees from ideal bond lengths and, bond angles, respectively. The final model contains 469 residue, 237 water, molecules, and one chloride ion. The segment between Trp182 and Asn192, could not be located in the electron density, nor could the N and C, termini. Cleavage of the calcium-free form of BLA was observed after, Glu189, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7877175 (full description)]]
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<StructureSection load='1bpl' size='340' side='right'caption='[[1bpl]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bpl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BPL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bpl OCA], [https://pdbe.org/1bpl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bpl RCSB], [https://www.ebi.ac.uk/pdbsum/1bpl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bpl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMY_BACLI AMY_BACLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bp/1bpl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bpl ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BPL is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BPL OCA]].
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*[[Amylase 3D structures|Amylase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution., Machius M, Wiegand G, Huber R, J Mol Biol. 1995 Mar 3;246(4):545-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7877175 7877175]
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[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Machius, M.]]
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[[Category: Machius M]]
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[[Category: Wiegand, G.]]
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[[Category: Wiegand G]]
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[[Category: 4-glucan-4-glucanohydrolase]]
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[[Category: alpha-1]]
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[[Category: alpha-amylase glycosyltransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:42:15 2007''
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Current revision

GLYCOSYLTRANSFERASE

PDB ID 1bpl

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