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1bro

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[[Image:1bro.gif|left|200px]]<br /><applet load="1bro" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bro, resolution 2.05&Aring;" />
 
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'''BROMOPEROXIDASE A2'''<br />
 
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==Overview==
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==BROMOPEROXIDASE A2==
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The crystal structure of the bromoperoxidase A2 from Streptomyces aureofaciens (ATCC 10762) has been determined by isomorphous replacement and refined to 2.05 A resolution with an R-value of 18.4%. The enzyme catalyzes the bromination of organic compounds in the presence of bromide and peroxide. The structure confirms the absence of cofactors such as metal ions or haem groups and shows the general topology of the alpha/beta hydrolase fold. The active centre is at the end of a deep pocket and includes a catalytic triad of Ser 98, Asp 228 and His 257. The active centre is connected by a narrow tunnel to a second pocket on the enzyme surface.
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<StructureSection load='1bro' size='340' side='right'caption='[[1bro]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BRO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bro OCA], [https://pdbe.org/1bro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bro RCSB], [https://www.ebi.ac.uk/pdbsum/1bro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bro ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BPOA2_KITAU BPOA2_KITAU] May be a chlorinating enzyme involved in 7-chlorotetracycline biosynthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/1bro_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bro ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BRO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Known structural/functional Sites: <scene name='pdbsite=ACT:Catalytic+Triad'>ACT</scene> and <scene name='pdbsite=BCT:Catalytic+Triad'>BCT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRO OCA].
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*[[Haloperoxidase|Haloperoxidase]]
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__TOC__
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==Reference==
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</StructureSection>
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The metal-ion-free oxidoreductase from Streptomyces aureofaciens has an alpha/beta hydrolase fold., Hecht HJ, Sobek H, Haag T, Pfeifer O, van Pee KH, Nat Struct Biol. 1994 Aug;1(8):532-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7664081 7664081]
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[[Category: Kitasatospora aureofaciens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Streptomyces aureofaciens]]
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[[Category: Haag T]]
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[[Category: Haag, T.]]
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[[Category: Hecht HJ]]
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[[Category: Hecht, H J.]]
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[[Category: Pfeifer O]]
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[[Category: Pee, K H.Van.]]
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[[Category: Sobek H]]
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[[Category: Pfeifer, O.]]
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[[Category: Van Pee KH]]
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[[Category: Sobek, H.]]
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[[Category: alpha/beta hydrolase fold]]
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[[Category: antibiotic biosynthesis]]
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[[Category: haloperoxidase]]
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[[Category: oxidoreductase]]
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[[Category: peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:20 2008''
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Current revision

BROMOPEROXIDASE A2

PDB ID 1bro

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