1cii

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1cii" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cii, resolution 3.0&Aring;" /> '''COLICIN IA'''<br /> ...)
Current revision (06:43, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1cii.gif|left|200px]]<br /><applet load="1cii" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1cii, resolution 3.0&Aring;" />
 
-
'''COLICIN IA'''<br />
 
-
==Overview==
+
==COLICIN IA==
-
The ion-channel forming colicins A, B, E1, Ia, Ib and N all kill bacterial, cells selectively by co-opting bacterial active-transport pathways and, forming voltage-gated ion conducting channels across the plasma membrane, of the target bacterium. The crystal structure of colicin Ia reveals a, molecule 210 A long with three distinct functional domains arranged along, a backbone of two extraordinarily long alpha-helices. A central domain at, the bend of the hairpin-like structure mediates specific recognition and, binding to an outer-membrane receptor. A second domain mediates, translocation across the outer membrane via the TonB transport pathway;, the TonB-box recognition element of colicin Ia is on one side of three 80, A-long helices arranged as a helical sheet. A third domain is made up of, 10 alpha-helices which form a voltage-activated and voltage-gated ion, conducting channel across the plasma membrane of the target cell. The two, 160 A-long alpha-helices that link the receptor-binding domain to the, other domains enable the colicin Ia molecule to span the periplasmic space, and contact both the outer and plasma membranes simultaneously during, function.
+
<StructureSection load='1cii' size='340' side='right'caption='[[1cii]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1cii]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CII FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cii OCA], [https://pdbe.org/1cii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cii RCSB], [https://www.ebi.ac.uk/pdbsum/1cii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cii ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CEIA_ECOLX CEIA_ECOLX] This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/1cii_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cii ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1CII is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CII OCA].
+
*[[Colicin|Colicin]]
-
 
+
*[[Colicin 3D structures|Colicin 3D structures]]
-
==Reference==
+
__TOC__
-
Crystal structure of colicin Ia., Wiener M, Freymann D, Ghosh P, Stroud RM, Nature. 1997 Jan 30;385(6615):461-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9009197 9009197]
+
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Freymann, D.]]
+
[[Category: Freymann D]]
-
[[Category: Ghosh, P.]]
+
[[Category: Ghosh P]]
-
[[Category: Stroud, R.]]
+
[[Category: Stroud R]]
-
[[Category: Wiener, M.]]
+
[[Category: Wiener M]]
-
[[Category: bacteriocin]]
+
-
[[Category: colicin]]
+
-
[[Category: ion channel formation]]
+
-
[[Category: transmembrane protein]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:29:19 2007''
+

Current revision

COLICIN IA

PDB ID 1cii

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cii"
Personal tools