This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1cqp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:44, 7 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1cqp.jpg|left|200px]]<br /><applet load="1cqp" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1cqp, resolution 2.60&Aring;" />
 
-
'''CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX LFA-1 (CD11A) I-DOMAIN / LOVASTATIN AT 2.6 A RESOLUTION'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX LFA-1 (CD11A) I-DOMAIN / LOVASTATIN AT 2.6 A RESOLUTION==
-
The lymphocyte function-associated antigen (LFA-1) belongs to the family, of beta2-integrins and plays an important role in T-cell activation and, leukocyte migration to sites of inflammation. We report here that, lovastatin, a drug clinically used for lowering cholesterol levels, inhibits the interaction of human LFA-1 with its counter-receptor, intercellular adhesion molecule-1. Using nuclear magnetic resonance, spectroscopy and X-ray crystallography we show that the inhibitor binds to, a highly conserved domain of the LFA-1 alpha-chain called the I-domain., The first three-dimensional structure of an integrin inhibitor bound to, its receptor reveals atomic details for a hitherto unknown mode of LFA-1, inhibition. It also sheds light into possible mechanisms of LFA-1 mediated, signalling and will support the design of novel anti-adhesive and, immunosuppressive drugs.
+
<StructureSection load='1cqp' size='340' side='right'caption='[[1cqp]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1cqp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQP FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=803:LOVASTATIN'>803</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqp OCA], [https://pdbe.org/1cqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cqp RCSB], [https://www.ebi.ac.uk/pdbsum/1cqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqp ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ITAL_HUMAN ITAL_HUMAN] Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/1cqp_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cqp ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1CQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=803:'>803</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQP OCA].
+
*[[Integrin 3D structures|Integrin 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structural basis for LFA-1 inhibition upon lovastatin binding to the CD11a I-domain., Kallen J, Welzenbach K, Ramage P, Geyl D, Kriwacki R, Legge G, Cottens S, Weitz-Schmidt G, Hommel U, J Mol Biol. 1999 Sep 10;292(1):1-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10493852 10493852]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Cottens, S.]]
+
[[Category: Cottens S]]
-
[[Category: Geyl, D.]]
+
[[Category: Geyl D]]
-
[[Category: Hommel, U.]]
+
[[Category: Hommel U]]
-
[[Category: Kallen, J.]]
+
[[Category: Kallen J]]
-
[[Category: Kriwacki, R.]]
+
[[Category: Kriwacki R]]
-
[[Category: Legge, G.]]
+
[[Category: Legge G]]
-
[[Category: Ramage, P.]]
+
[[Category: Ramage P]]
-
[[Category: Weitz-Schmidt, G.]]
+
[[Category: Weitz-Schmidt G]]
-
[[Category: Welzenbach, K.]]
+
[[Category: Welzenbach K]]
-
[[Category: 803]]
+
-
[[Category: MG]]
+
-
[[Category: rossman fold]]
+
-
[[Category: structural basis for lfa-1 inhibition]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:36:45 2008''
+

Current revision

CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX LFA-1 (CD11A) I-DOMAIN / LOVASTATIN AT 2.6 A RESOLUTION

PDB ID 1cqp

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cqp"
Personal tools