1d03

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[[Image:1d03.jpg|left|200px]]
 
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==REFINED STRUCTURES OF OXIDIZED FLAVODOXIN FROM ANACYSTIS NIDULANS==
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The line below this paragraph, containing "STRUCTURE_1d03", creates the "Structure Box" on the page.
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<StructureSection load='1d03' size='340' side='right'caption='[[1d03]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1d03]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D03 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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{{STRUCTURE_1d03| PDB=1d03 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d03 OCA], [https://pdbe.org/1d03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d03 RCSB], [https://www.ebi.ac.uk/pdbsum/1d03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d03 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FLAV_SYNE7 FLAV_SYNE7] Low-potential electron donor to a number of redox enzymes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/1d03_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d03 ConSurf].
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<div style="clear:both"></div>
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'''REFINED STRUCTURES OF OXIDIZED FLAVODOXIN FROM ANACYSTIS NIDULANS'''
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==See Also==
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*[[Flavodoxin 3D structures|Flavodoxin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Flavodoxin from Anacystis nidulans (Synechococcus PCC 7942) was the first member of the flavodoxin family to be characterized, and is the structural prototype for the "long-chain" flavodoxins that have molecular masses of approximately 20 kDa. Crystal structure analyses and refinements of three orthorhombic forms of oxidized A. nidulans flavodoxin are reported, and salient features of the fold and the FMN binding site are compared with other flavodoxins. The structure of form I (wild-type: P212121, a=57.08 A, b=69.24 A, c=45.55 A), determined initially by multiple isomorphous replacement, has been refined to R=0.183 and R(free)=0.211 for data from 10.0 to 1.7 A resolution. Structures of form II (wild-type: P212121, a=60.05 A, b=65.85 A, c=51.36 A) and form III (Asn58Gly: P212121, a=51.30 A, b=59.15 A, c=94.44 A) have been determined by molecular replacement and refined versus data to 2.0 A and 1.85 A, respectively; the R values for forms II and III are 0.147 and 0.150. Changes in the molecular contacts that produce the alternative packings in these crystalline forms are analyzed. Deletion of the Asn side-chain in the mutant Asn58Gly removes an intermolecular stacking interaction and allows the alternative packing found in form III crystals. The functionally important 50's loop of the FMN binding site is less restrained by intermolecular contacts in these crystals but maintains the same conformation as in oxidized wild type protein. The structures reported here provide the starting point for structure-function studies of the reduced states and of mutants, described in the accompanying paper.
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[[Category: Large Structures]]
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[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
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==About this Structure==
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[[Category: Drennan CL]]
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1D03 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D03 OCA].
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[[Category: Hoover DM]]
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[[Category: Ludwig ML]]
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==Reference==
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[[Category: Metzger AL]]
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Refined structures of oxidized flavodoxin from Anacystis nidulans., Drennan CL, Pattridge KA, Weber CH, Metzger AL, Hoover DM, Ludwig ML, J Mol Biol. 1999 Dec 3;294(3):711-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10610791 10610791]
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[[Category: Pattridge KA]]
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[[Category: Single protein]]
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[[Category: Weber CH]]
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[[Category: Synechococcus sp.]]
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[[Category: Drennan, C L.]]
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[[Category: Hoover, D M.]]
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[[Category: Ludwig, M L.]]
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[[Category: Metzger, A L.]]
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[[Category: Pattridge, K A.]]
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[[Category: Weber, C H.]]
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[[Category: Flavodoxin]]
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[[Category: Fmn binding]]
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[[Category: Redox potential]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:17:46 2008''
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Current revision

REFINED STRUCTURES OF OXIDIZED FLAVODOXIN FROM ANACYSTIS NIDULANS

PDB ID 1d03

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