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1d8x

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[[Image:1d8x.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G A BASE PAIRS==
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|PDB= 1d8x |SIZE=350|CAPTION= <scene name='initialview01'>1d8x</scene>, resolution 1.20&Aring;
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<StructureSection load='1d8x' size='340' side='right'caption='[[1d8x]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE+ION'>NCO</scene>
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<table><tr><td colspan='2'>[[1d8x]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D8X FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d8x OCA], [https://pdbe.org/1d8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d8x RCSB], [https://www.ebi.ac.uk/pdbsum/1d8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d8x ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d8x OCA], [http://www.ebi.ac.uk/pdbsum/1d8x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d8x RCSB]</span>
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__TOC__
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}}
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</StructureSection>
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[[Category: Large Structures]]
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'''CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G A BASE PAIRS'''
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[[Category: Gao Y-G]]
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[[Category: Joachimiak A]]
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[[Category: Robinson H]]
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==Overview==
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[[Category: Sanishvili R]]
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G x A mismatched base pairs are frequently found in nucleic acids. Human centromere DNA sequences contain unusual repeating motifs, e.g. , (GAATG)n x (CATTC)n found in the human chromosome. The purine-rich strand of this repeating pentamer sequence forms duplex and hairpin structures with unusual stability. The high stability of these structures is contributed by the "sheared" G x A base pairs which present a novel recognition surface for ligands and proteins. We have solved the crystal structure, by the multiple-wavelength anomalous diffraction (MAD) method of d(CCGAATGAGG) in which the centromere core sequence motif GAATG is embedded. Three crystal forms were refined to near-atomic resolution. The structures reveal the detailed conformation of tandem G x A base pairs whose unique hydrogen-bonding surface has interesting interactions with bases, hydrated magnesium ions, cobalt(III)hexaammine, spermine, and water molecules. The results are relevant in understanding the structure associated with human centromere sequence in particular and G x A base pairs in nucleic acids (including RNA, like ribozyme) in general.
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[[Category: Wang AH-J]]
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==About this Structure==
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1D8X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8X OCA].
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==Reference==
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Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10600106 10600106]
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[[Category: Protein complex]]
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[[Category: Gao, Y G.]]
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[[Category: Joachimiak, A.]]
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[[Category: Robinson, H.]]
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[[Category: Sanishvili, R.]]
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[[Category: Wang, A H.J.]]
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[[Category: ga mismatch]]
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[[Category: tandem ga base pair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:37:05 2008''
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Current revision

CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G A BASE PAIRS

PDB ID 1d8x

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