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| - | [[Image:1da1.gif|left|200px]] | |
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| - | {{Structure
| + | ==STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT== |
| - | |PDB= 1da1 |SIZE=350|CAPTION= <scene name='initialview01'>1da1</scene>, resolution 2.250Å
| + | <StructureSection load='1da1' size='340' side='right'caption='[[1da1]], [[Resolution|resolution]] 2.25Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=BRU:5-BROMO-2'-DEOXYURIDINE-5'-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
| + | <table><tr><td colspan='2'>[[1da1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DA1 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1da1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1da1 OCA], [https://pdbe.org/1da1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1da1 RCSB], [https://www.ebi.ac.uk/pdbsum/1da1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1da1 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1da1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1da1 OCA], [http://www.ebi.ac.uk/pdbsum/1da1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1da1 RCSB]</span>
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| - | }}
| + | |
| | | | |
| - | '''STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT'''
| + | ==See Also== |
| - | | + | *[[Z-DNA|Z-DNA]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | The deoxyoligonucleotide d(BrU-G-C-G-C-G) was crystallised at pH 8.2 and its structure analysed by X-ray diffraction. The unit cell, of dimensions a = 17.94, b = 30.85, c = 49.94A contains four DNA duplexes in space group P2(1)2(1)2(1). The duplexes are in the Z conformation, with four Watson-Crick G.C base pairs and two BrU.G base pairs. The structure was refined to an R factor of 0.16 at a resolution of 2.2A with 64 solvent molecules located. The BrU.G base pair mismatch is of the wobble type, with both bases in the major tautomer form and hydrogen bonds linking 0-2 of BrU with N-1 of G and N3 of BrU with 0-6 of G. There is no indication of the presence of ionised base pairs, in spite of the high pH of crystallisation. The results are discussed in terms of the mutagenic properties of 5- bromouracil.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Brown T]] |
| - | ==About this Structure==
| + | [[Category: Hunter WN]] |
| - | 1DA1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DA1 OCA].
| + | [[Category: Kennard O]] |
| - | | + | [[Category: Kneale G]] |
| - | ==Reference==
| + | |
| - | Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3951996 3951996]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Brown, T.]] | + | |
| - | [[Category: Hunter, W N.]] | + | |
| - | [[Category: Kennard, O.]] | + | |
| - | [[Category: Kneale, G.]] | + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: mismatched]]
| + | |
| - | [[Category: modified]]
| + | |
| - | [[Category: z-dna]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:37:39 2008''
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