1dco

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(New page: 200px<br /><applet load="1dco" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dco, resolution 2.3&Aring;" /> '''DCOH, A BIFUNCTIONAL ...)
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[[Image:1dco.jpg|left|200px]]<br /><applet load="1dco" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dco, resolution 2.3&Aring;" />
 
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'''DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR'''<br />
 
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==Overview==
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==DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR==
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DCoH, the dimerization cofactor of hepatocyte nuclear factor 1 (HNF-1), functions as both a transcriptional coactivator and a pterin dehydratase., To probe the relationship between these two functions, the X-ray crystal, structures of the free enzyme and its complex with the product analogue, 7,8-dihydrobiopterin were refined at 2.3 A resolution. The ligand binds at, four sites per tetrameric enzyme, with little apparent conformational, change in the protein. Each active-site cleft is located in a subunit, interface, adjacent to a prominent saddle motif that has structural, similarities to the TATA binding protein. The pterin binds within an arch, of aromatic residues that extends across one dimer interface. The bound, ligand makes contacts to three conserved histidines, and this arrangement, restricts proposals for the enzymatic mechanism of dehydration. The, dihedral symmetry of DCoH suggests that binding to the dimerization domain, of HNF-1 likely involves the superposition of two-fold rotation axes of, the two proteins.
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<StructureSection load='1dco' size='340' side='right'caption='[[1dco]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1dco]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DCO FirstGlance]. <br>
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1DCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Active as [http://en.wikipedia.org/wiki/4a-hydroxytetrahydrobiopterin_dehydratase 4a-hydroxytetrahydrobiopterin dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.96 4.2.1.96] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DCO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dco OCA], [https://pdbe.org/1dco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dco RCSB], [https://www.ebi.ac.uk/pdbsum/1dco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dco ProSAT]</span></td></tr>
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==Reference==
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</table>
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High-resolution structures of the bifunctional enzyme and transcriptional coactivator DCoH and its complex with a product analogue., Cronk JD, Endrizzi JA, Alber T, Protein Sci. 1996 Oct;5(10):1963-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8897596 8897596]
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== Function ==
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[[Category: 4a-hydroxytetrahydrobiopterin dehydratase]]
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[https://www.uniprot.org/uniprot/PHS_RAT PHS_RAT] Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.<ref>PMID:1763325</ref> <ref>PMID:8444860</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dc/1dco_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dco ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Alber T]]
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[[Category: Alber, T.]]
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[[Category: Cronk JD]]
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[[Category: Cronk, J.D.]]
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[[Category: Endrizzi JA]]
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[[Category: Endrizzi, J.A.]]
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[[Category: 4a-carbinolamine dehydratase]]
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[[Category: dehydratase]]
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[[Category: dimerization cofactor]]
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[[Category: transcriptional stimulator]]
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[[Category: transregulator of homeodomain proteins]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:10:32 2007''
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Current revision

DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR

PDB ID 1dco

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