1dcs

<StructureSection load='1dcs' size='340' side='right' caption='[[1dcs]], [[Resolution|resolution]] 1.30&Aring;' scene=''>

<table><tr><td colspan='2'>[[1dcs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DCS FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dcs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dcs RCSB], [http://www.ebi.ac.uk/pdbsum/1dcs PDBsum]</span></td></tr>

[[http://www.uniprot.org/uniprot/CEFE_STRCL CEFE_STRCL]] Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dc/1dcs_consurf.spt"</scriptWhenChecked>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].

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== Publication Abstract from PubMed ==

Penicillins and cephalosporins are among the most widely used therapeutic agents. These antibiotics are produced from fermentation-derived materials as their chemical synthesis is not commercially viable. Unconventional steps in their biosynthesis are catalysed by Fe(II)-dependent oxidases/oxygenases; isopenicillin N synthase (IPNS) creates in one step the bicyclic nucleus of penicillins, and deacetoxycephalosporin C synthase (DAOCS) catalyses the expansion of the penicillin nucleus into the nucleus of cephalosporins. Both enzymes use dioxygen-derived ferryl intermediates in catalysis but, in contrast to IPNS, the ferryl form of DAOCS is produced by the oxidative splitting of a co-substrate, 2-oxoglutarate (alpha-ketoglutarate). This route of controlled ferryl formation and reaction is common to many mononuclear ferrous enzymes, which participate in a broader range of reactions than their well-characterized counterparts, the haem enzymes. Here we report the first crystal structure of a 2-oxoacid-dependent oxygenase. High-resolution structures for apo-DAOCS, the enzyme complexed with Fe(II), and with Fe(II) and 2-oxoglutarate, were obtained from merohedrally twinned crystals. Using a model based on these structures, we propose a mechanism for ferryl formation.

Structure of a cephalosporin synthase.,Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S, Perrakis A, Thompson A, Lee HJ, Baldwin JE, Schofield CJ, Hajdu J, Andersson I Nature. 1998 Aug 20;394(6695):805-9. PMID:9723623<ref>PMID:9723623</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Andersson, I]]

[[Category: Baldwin, J E]]

[[Category: Hajdu, J]]

[[Category: Hara, T]]

[[Category: Lee, H J]]

[[Category: Lloyd, M D]]

[[Category: Perrakis, A]]

[[Category: Ramaswamy, S]]

[[Category: Scheltinga, A C.Terwisscha Van]]

[[Category: Schofield, C J]]

[[Category: Thompson, A]]

[[Category: Valegard, K]]

[[Category: Oxidoreductase]]