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1dp9

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[[Image:1dp9.gif|left|200px]]<br /><applet load="1dp9" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dp9, resolution 2.6&Aring;" />
 
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'''CRYSTAL STRUCTURE OF IMIDAZOLE-BOUND FIXL HEME DOMAIN'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF IMIDAZOLE-BOUND FIXL HEME DOMAIN==
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The FixL heme domain serves as the dioxygen switch in the FixL/FixJ two-component system of Rhizobia. Recent structural studies of the Bradyrhizobium japonicum FixL heme domain (BjFixLH) have suggested an allosteric mechanism that is distinct from the classical hemoglobin model. To gain further insight into the FixL sensing mechanism, structures of BjFixLH bound to dioxygen, imidazole, and nitric oxide have been determined. These structures, particularly the structure of BjFixLH bound to its physiological ligand, dioxygen, have helped to address a number of important issues relevant to the BjFixLH sensing mechanism. On the basis of the oxy-BjFixLH structure, a conserved arginine is found to stabilize the dioxygen ligand in a mode reminiscent of the distal histidine in classical myoglobins and hemoglobins. The structure of BjFixLH bound to imidazole elucidates the structural requirements for accommodating sterically bulky ligands. Finally, the structure of BjFixLH bound to nitric oxide provides evidence for a structural intermediate in the heme-driven conformational change.
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<StructureSection load='1dp9' size='340' side='right'caption='[[1dp9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1dp9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DP9 FirstGlance]. <br>
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1DP9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum] with <scene name='pdbligand=IMD:'>IMD</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DP9 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dp9 OCA], [https://pdbe.org/1dp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dp9 RCSB], [https://www.ebi.ac.uk/pdbsum/1dp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dp9 ProSAT]</span></td></tr>
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New mechanistic insights from structural studies of the oxygen-sensing domain of Bradyrhizobium japonicum FixL., Gong W, Hao B, Chan MK, Biochemistry. 2000 Apr 11;39(14):3955-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10747783 10747783]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FIXL_BRADU FIXL_BRADU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/1dp9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dp9 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bradyrhizobium japonicum]]
[[Category: Bradyrhizobium japonicum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chan, M K.]]
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[[Category: Chan MK]]
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[[Category: Gong, W.]]
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[[Category: Gong W]]
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[[Category: Hao, B.]]
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[[Category: Hao B]]
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[[Category: HEM]]
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[[Category: IMD]]
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[[Category: fixl heme domain liganded structure]]
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[[Category: heme]]
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[[Category: histidine kinase]]
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[[Category: pas domain family]]
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[[Category: two component system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:18:57 2008''
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Current revision

CRYSTAL STRUCTURE OF IMIDAZOLE-BOUND FIXL HEME DOMAIN

PDB ID 1dp9

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