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1dt0

From Proteopedia

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(New page: 200px<br /><applet load="1dt0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dt0, resolution 2.1&Aring;" /> '''CLONING, SEQUENCE, AN...)
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[[Image:1dt0.gif|left|200px]]<br /><applet load="1dt0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dt0, resolution 2.1&Aring;" />
 
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'''CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS'''<br />
 
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==Overview==
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==CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS==
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The gene encoding the iron-dependent superoxide dismutase from Pseudomonas, ovalis was cloned from a genomic library and sequenced. The ORF differs, from the previously published protein sequence, which was used for the, original structure determination, at 16 positions. The differences include, three additional inserted residues, one deleted residue and 12 point, substitutions. The gene was subcloned and the recombinant protein, overexpressed, purified and crystallized in a trigonal space group. The, structure was determined by molecular replacement and was refined to 2.1 A, resolution.
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<StructureSection load='1dt0' size='340' side='right'caption='[[1dt0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dt0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DT0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dt0 OCA], [https://pdbe.org/1dt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dt0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dt0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SODF_PSEPU SODF_PSEPU] Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/1dt0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dt0 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DT0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with FE as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DT0 OCA].
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*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis., Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11053832 11053832]
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[[Category: Large Structures]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: Single protein]]
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[[Category: Bond CJ]]
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[[Category: Superoxide dismutase]]
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[[Category: Flick K]]
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[[Category: Bond, C.J.]]
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[[Category: Hajduk R]]
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[[Category: Flick, K.]]
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[[Category: Heath P]]
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[[Category: Hajduk, R.]]
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[[Category: Huang J]]
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[[Category: Heath, P.]]
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[[Category: Stoddard BL]]
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[[Category: Huang, J.]]
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[[Category: Stoddard, B.L.]]
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[[Category: FE]]
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[[Category: pseudomonas ovalis]]
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[[Category: superoxide dismutase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:33:30 2007''
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Current revision

CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS

PDB ID 1dt0

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