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1efw

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(New page: 200px<br /><applet load="1efw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1efw, resolution 3.00&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1efw.gif|left|200px]]<br /><applet load="1efw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1efw, resolution 3.00&Aring;" />
 
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'''CRYSTAL STRUCTURE OF ASPARTYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED TO TRNAASP FROM ESCHERICHIA COLI'''<br />
 
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==Overview==
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==Crystal structure of aspartyl-tRNA synthetase from Thermus thermophilus complexed to tRNAasp from Escherichia coli==
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The crystal structures of aspartyl-tRNA synthetase (AspRS) from Thermus, thermophilus, a prokaryotic class IIb enzyme, complexed with tRNA(Asp), from either T. thermophilus or Escherichia coli reveal a potential, intermediate of the recognition process. The tRNA is positioned on the, enzyme such that it cannot be aminoacylated but adopts an overall, conformation similar to that observed in active complexes. While the, anticodon loop binds to the N-terminal domain of the enzyme in a manner, similar to that of the related active complexes, its aminoacyl acceptor, arm remains at the entrance of the active site, stabilized in its, intermediate conformational state by non-specific interactions with the, insertion and catalytic domains. The thermophilic nature of the enzyme, which manifests itself in a very low kinetic efficiency at 17 degrees C, the temperature at which the crystals were grown, is in agreement with the, relative stability of this non-productive conformational state. Based on, these data, a pathway for tRNA binding and recognition is proposed.
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<StructureSection load='1efw' size='340' side='right'caption='[[1efw]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1efw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EFW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2MA:2-METHYLADENOSINE-5-MONOPHOSPHATE'>2MA</scene>, <scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=G7M:N7-METHYL-GUANOSINE-5-MONOPHOSPHATE'>G7M</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=QUO:2-AMINO-7-DEAZA-(2,3-DIHYDROXY-CYCLOPENTYLAMINO)-GUANOSINE-5-MONOPHOSPHATE'>QUO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1efw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1efw OCA], [https://pdbe.org/1efw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1efw RCSB], [https://www.ebi.ac.uk/pdbsum/1efw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1efw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYD_THETH SYD_THETH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/1efw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1efw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EFW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Active as [http://en.wikipedia.org/wiki/Aspartate--tRNA_ligase Aspartate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.12 6.1.1.12] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EFW OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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==Reference==
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__TOC__
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An intermediate step in the recognition of tRNA(Asp) by aspartyl-tRNA synthetase., Briand C, Poterszman A, Eiler S, Webster G, Thierry J, Moras D, J Mol Biol. 2000 Jun 16;299(4):1051-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10843857 10843857]
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</StructureSection>
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[[Category: Aspartate--tRNA ligase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Briand, C.]]
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[[Category: Briand C]]
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[[Category: Eiler, S.]]
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[[Category: Eiler S]]
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[[Category: Moras, D.]]
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[[Category: Moras D]]
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[[Category: Poterszman, A.]]
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[[Category: Poterszman A]]
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[[Category: Thierry, J.C.]]
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[[Category: Thierry J-C]]
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[[Category: Webster, G.]]
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[[Category: Webster G]]
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[[Category: aspartyl-trna synthetase]]
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[[Category: ligase/rna]]
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[[Category: protein/rna complex]]
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[[Category: trna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:58:12 2007''
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Current revision

Crystal structure of aspartyl-tRNA synthetase from Thermus thermophilus complexed to tRNAasp from Escherichia coli

PDB ID 1efw

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