This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1egy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:02, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1egy.gif|left|200px]]
 
-
{{Structure
+
==CYTOCHROME P450ERYF WITH 9-AMINOPHENANTHRENE BOUND==
-
|PDB= 1egy |SIZE=350|CAPTION= <scene name='initialview01'>1egy</scene>, resolution 2.35&Aring;
+
<StructureSection load='1egy' size='340' side='right'caption='[[1egy]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=9AP:9-AMINOPHENANTHRENE'>9AP</scene>
+
<table><tr><td colspan='2'>[[1egy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EGY FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9AP:9-AMINOPHENANTHRENE'>9AP</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1egy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1egy OCA], [https://pdbe.org/1egy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1egy RCSB], [https://www.ebi.ac.uk/pdbsum/1egy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1egy ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CPXJ_SACEN CPXJ_SACEN] Catalyzes the NADPH-dependent conversion of 6-deoxyerythronolide B (6-DEB) to erythronolide B (EB) by the insertion of an oxygen at the 6S position of 6-DEB. Requires the participation of a ferredoxin and a ferredoxin reductase for the transfer of electrons from NADPH to the monooxygenase.<ref>PMID:1732208</ref> <ref>PMID:2011746</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1egy_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1egy ConSurf].
 +
<div style="clear:both"></div>
-
'''CYTOCHROME P450ERYF WITH 9-AMINOPHENANTHRENE BOUND'''
+
==See Also==
-
 
+
*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
-
 
+
== References ==
-
==Overview==
+
<references/>
-
Several mammalian cytochrome P450 (P450) isoforms demonstrate homotropic cooperativity with a number of substrates, including steroids and polycyclic aromatic hydrocarbons. To identify structural factors contributing to steroid and polycyclic aromatic hydrocarbon binding to P450 enzymes and to determine the location of the allosteric site, we investigated interactions of the macrolide hydroxylase P450eryF from Saccharopolyspora erythraea with androstenedione and 9-aminophenanthrene. Spectroscopic binding assays indicate that P450eryF binds androstenedione with an affinity of 365 microM and a Hill coefficient of 1.31 +/- 0.6 and coordinates with 9-aminophenanthrene with an affinity of 91 microM and a Hill coefficient of 1.38 +/- 0.2. Crystals of complexes of androstenedione and 9-aminophenanthrene with P450eryF were grown and diffracted to 2.1 A and 2.35 A, respectively. Electron density maps indicate that for both complexes two ligand molecules are simultaneously present in the active site. The P450eryF/androstenedione model was refined to an r = 18.9%, and the two androstenedione molecules have similar conformations. The proximal androstenedione is positioned such that the alpha-face of carbon-6 is closest to the heme iron, and the second steroid molecule is positioned 5.5 A distal in the active site. The P450eryF/9-aminophenanthrene model was refined to an r = 19.7% with the proximal 9-aminophenanthrene coordinated with the heme iron through the 9-amino group and the second ligand positioned approximately 6 A distal in the active site. These results establish that homotropic cooperativity in ligand binding can result from binding of two substrate molecules within the active site pocket without major conformational changes in the protein.
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
1EGY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGY OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structures of ligand complexes of P450eryF exhibiting homotropic cooperativity., Cupp-Vickery J, Anderson R, Hatziris Z, Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3050-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10716705 10716705]
+
[[Category: Saccharopolyspora erythraea]]
[[Category: Saccharopolyspora erythraea]]
-
[[Category: Single protein]]
+
[[Category: Anderson R]]
-
[[Category: Anderson, R.]]
+
[[Category: Cupp-Vickery JR]]
-
[[Category: Cupp-Vickery, J R.]]
+
[[Category: Hatziris Z]]
-
[[Category: Hatziris, Z.]]
+
-
[[Category: 9AP]]
+
-
[[Category: HEM]]
+
-
[[Category: cytochrome p450 oxidoreductase polycyclic aromatic hydrocarbon p450eryf]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:55:31 2008''
+

Current revision

CYTOCHROME P450ERYF WITH 9-AMINOPHENANTHRENE BOUND

PDB ID 1egy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1egy"
Personal tools