1eiz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1eiz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eiz, resolution 1.7&Aring;" /> '''FTSJ RNA METHYLTRANSF...)
Current revision (07:03, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1eiz.jpg|left|200px]]<br /><applet load="1eiz" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1eiz, resolution 1.7&Aring;" />
 
-
'''FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE'''<br />
 
-
==Overview==
+
==FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE==
-
Structural, biochemical, and genetic techniques were applied to, investigate the function of FtsJ, a recently identified heat shock, protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A, crystal structure of FtsJ in complex with its cofactor, S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The, molecular surface of FtsJ exposes a putative nucleic acid binding groove, composed of highly conserved, positively charged residues. Substrate, analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal, subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically, altered ribosome profile, a severe growth disadvantage, and a, temperature-sensitive phenotype. Our results reveal an unexpected link, between the heat shock response and RNA metabolism.
+
<StructureSection load='1eiz' size='340' side='right'caption='[[1eiz]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1eiz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIZ FirstGlance]. <br>
-
1EIZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SAM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EIZ OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eiz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eiz OCA], [https://pdbe.org/1eiz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eiz RCSB], [https://www.ebi.ac.uk/pdbsum/1eiz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eiz ProSAT]</span></td></tr>
-
RNA methylation under heat shock control., Bugl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U, Mol Cell. 2000 Aug;6(2):349-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10983982 10983982]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RLME_ECOLI RLME_ECOLI] Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.<ref>PMID:10748051</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eiz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eiz ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bardwell, J.C.A.]]
+
[[Category: Bardwell JCA]]
-
[[Category: Bugl, H.]]
+
[[Category: Bugl H]]
-
[[Category: Fauman, E.B.]]
+
[[Category: Fauman EB]]
-
[[Category: Jakob, U.]]
+
[[Category: Jakob U]]
-
[[Category: Kushner, S.R.]]
+
[[Category: Kushner SR]]
-
[[Category: Saper, M.A.]]
+
[[Category: Saper MA]]
-
[[Category: Staker, B.L.]]
+
[[Category: Staker BL]]
-
[[Category: Zheng, F.]]
+
[[Category: Zheng F]]
-
[[Category: SAM]]
+
-
[[Category: 23s ribosomal rna]]
+
-
[[Category: adenosyl methionine]]
+
-
[[Category: adomet]]
+
-
[[Category: ftsj]]
+
-
[[Category: heat shock proteins]]
+
-
[[Category: methyltransferase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:02:18 2007''
+

Current revision

FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE

PDB ID 1eiz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1eiz"
Personal tools