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1elp

From Proteopedia

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[[Image:1elp.gif|left|200px]]<br /><applet load="1elp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1elp, resolution 1.95&Aring;" />
 
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'''GAMMA-D CRYSTALLIN STRUCTURE AT 1.95 A RESOLUTION'''<br />
 
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==Overview==
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==GAMMA-D CRYSTALLIN STRUCTURE AT 1.95 A RESOLUTION==
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The crystal structure of bovine lens gammaIIIb-crystallin at 2.5 A resolution previously reported was interpreted using a consensus sequence derived from related vertebrate sequences on the assumption that gammaIIIb-crystallin derived from the gammaC-crystallin gene. It has recently been shown that gammaIIIb is a product of the bovine gammaD gene. The structure of gammaIIIb has now been refined with the bovine gammaD sequence using new 1.95 A resolution synchrotron data. The crystallographic R factor was 20.4% for all 33 104 reflection data between 8.0 and 1.95 A measured at 277(1) K. The electron density fully supported the assignment of the gammaD sequence to gammaIIIb. The crystal belongs to space group P2(1)2(1)2(1) with two molecules of molecular mass 20 749 Da in the asymmetric unit in which 219 water molecules were located. The two-domain four-Greek-key motif highly symmetrical protein is very similar in structure to gammaB-crystallin (81% sequence identity). There is a single amino-acid deletion in gammaD in the linker region connecting the two domains. The intermolecular oganization in the crystal lattice is quite different from gammaB as a result of key mutations involving surface residues Leu51, Ile103 and His155. These point mutations will contribute to the intermolecular behaviour of the gamma-crystallins in the eye lens, where they are major components of the densely packed, high refractive index regions of the lens.
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<StructureSection load='1elp' size='340' side='right'caption='[[1elp]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1elp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ELP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1elp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elp OCA], [https://pdbe.org/1elp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1elp RCSB], [https://www.ebi.ac.uk/pdbsum/1elp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1elp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CRGD_BOVIN CRGD_BOVIN] Crystallins are the dominant structural components of the vertebrate eye lens.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1elp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1elp ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ELP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELP OCA].
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*[[Crystallin 3D structures|Crystallin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of bovine eye lens gammaD (gammaIIIb)-crystallin at 1.95 A., Chirgadze YN, Driessen HP, Wright G, Slingsby C, Hay RE, Lindley PF, Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):712-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299634 15299634]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chirgadze, Yu N.]]
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[[Category: Chirgadze YuN]]
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[[Category: Driessen, H P.C.]]
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[[Category: Driessen HPC]]
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[[Category: Hay, R E.]]
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[[Category: Hay RE]]
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[[Category: Lindley, P F.]]
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[[Category: Lindley PF]]
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[[Category: Slingsby, C.]]
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[[Category: Slingsby C]]
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[[Category: Wright, G.]]
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[[Category: Wright G]]
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[[Category: eye lens protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:04 2008''
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Current revision

GAMMA-D CRYSTALLIN STRUCTURE AT 1.95 A RESOLUTION

PDB ID 1elp

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