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1eof

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(New page: 200px<br /><applet load="1eof" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eof, resolution 2.38&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1eof.jpg|left|200px]]<br /><applet load="1eof" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eof, resolution 2.38&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN==
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The T1 domain, a highly conserved cytoplasmic portion at the N-terminus of, the voltage-dependent K+ channel (Kv) alpha-subunit, is responsible for, driving and regulating the tetramerization of the alpha-subunits. Here we, report the identification of a set of mutations in the T1 domain that, alter the gating properties of the Kv channel. Two mutants produce a, leftward shift in the activation curve and slow the channel closing rate, while a third mutation produces a rightward shift in the activation curve, and speeds the channel closing rate. We have determined the crystal, structures of T1 domains containing these mutations. Both of the leftward, shifting mutants produce similar conformational changes in the putative, membrane facing surface of the T1 domain. These results suggest that the, structure of the T1 domain in this region is tightly coupled to the, channel's gating states.
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<StructureSection load='1eof' size='340' side='right'caption='[[1eof]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eof]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eof OCA], [https://pdbe.org/1eof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eof RCSB], [https://www.ebi.ac.uk/pdbsum/1eof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eof ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q16968_APLCA Q16968_APLCA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eof_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eof ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EOF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EOF OCA].
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Voltage dependent activation of potassium channels is coupled to T1 domain structure., Cushman SJ, Nanao MH, Jahng AW, DeRubeis D, Choe S, Pfaffinger PJ, Nat Struct Biol. 2000 May;7(5):403-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10802739 10802739]
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[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Choe, S.]]
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[[Category: Choe S]]
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[[Category: Cushman, S.J.]]
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[[Category: Cushman SJ]]
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[[Category: DeRubeis, D.]]
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[[Category: DeRubeis D]]
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[[Category: Jahng, A.W.]]
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[[Category: Jahng AW]]
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[[Category: Nanao, M.H.]]
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[[Category: Nanao MH]]
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[[Category: Pfaffinger, P.J.]]
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[[Category: Pfaffinger PJ]]
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[[Category: aplysia kv1.1]]
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[[Category: potassium channels]]
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[[Category: proton transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:37:06 2007''
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Current revision

CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN

PDB ID 1eof

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