1eup

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(New page: 200px<br /><applet load="1eup" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eup, resolution 2.1&Aring;" /> '''X-RAY CRYSTAL STRUCTU...)
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[[Image:1eup.gif|left|200px]]<br /><applet load="1eup" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eup, resolution 2.1&Aring;" />
 
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'''X-RAY CRYSTAL STRUCTURE OF CYTOCHROME P450ERYF WITH ANDROSTENDIONE BOUND'''<br />
 
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==Overview==
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==X-RAY CRYSTAL STRUCTURE OF CYTOCHROME P450ERYF WITH ANDROSTENDIONE BOUND==
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Several mammalian cytochrome P450 (P450) isoforms demonstrate homotropic, cooperativity with a number of substrates, including steroids and, polycyclic aromatic hydrocarbons. To identify structural factors, contributing to steroid and polycyclic aromatic hydrocarbon binding to, P450 enzymes and to determine the location of the allosteric site, we, investigated interactions of the macrolide hydroxylase P450eryF from, Saccharopolyspora erythraea with androstenedione and 9-aminophenanthrene., Spectroscopic binding assays indicate that P450eryF binds androstenedione, with an affinity of 365 microM and a Hill coefficient of 1.31 +/- 0.6 and, coordinates with 9-aminophenanthrene with an affinity of 91 microM and a, Hill coefficient of 1.38 +/- 0.2. Crystals of complexes of androstenedione, and 9-aminophenanthrene with P450eryF were grown and diffracted to 2.1 A, and 2.35 A, respectively. Electron density maps indicate that for both, complexes two ligand molecules are simultaneously present in the active, site. The P450eryF/androstenedione model was refined to an r = 18.9%, and, the two androstenedione molecules have similar conformations. The proximal, androstenedione is positioned such that the alpha-face of carbon-6 is, closest to the heme iron, and the second steroid molecule is positioned, 5.5 A distal in the active site. The P450eryF/9-aminophenanthrene model, was refined to an r = 19.7% with the proximal 9-aminophenanthrene, coordinated with the heme iron through the 9-amino group and the second, ligand positioned approximately 6 A distal in the active site. These, results establish that homotropic cooperativity in ligand binding can, result from binding of two substrate molecules within the active site, pocket without major conformational changes in the protein.
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<StructureSection load='1eup' size='340' side='right'caption='[[1eup]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eup]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eh0 1eh0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EUP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASD:4-ANDROSTENE-3-17-DIONE'>ASD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eup OCA], [https://pdbe.org/1eup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eup RCSB], [https://www.ebi.ac.uk/pdbsum/1eup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eup ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPXJ_SACEN CPXJ_SACEN] Catalyzes the NADPH-dependent conversion of 6-deoxyerythronolide B (6-DEB) to erythronolide B (EB) by the insertion of an oxygen at the 6S position of 6-DEB. Requires the participation of a ferredoxin and a ferredoxin reductase for the transfer of electrons from NADPH to the monooxygenase.<ref>PMID:1732208</ref> <ref>PMID:2011746</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/1eup_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eup ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EUP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with HEM and ASD as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1EH0. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EUP OCA].
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structures of ligand complexes of P450eryF exhibiting homotropic cooperativity., Cupp-Vickery J, Anderson R, Hatziris Z, Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3050-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10716705 10716705]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Anderson, R.]]
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[[Category: Large Structures]]
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[[Category: Cupp-Vickery, J.R.]]
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[[Category: Saccharopolyspora erythraea]]
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[[Category: Hatziris, Z.]]
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[[Category: Anderson R]]
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[[Category: ASD]]
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[[Category: Cupp-Vickery JR]]
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[[Category: HEM]]
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[[Category: Hatziris Z]]
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[[Category: androstenedione]]
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[[Category: cytochrome p450]]
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[[Category: cytochrome p450eryf]]
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[[Category: steroid]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:20:00 2007''
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Current revision

X-RAY CRYSTAL STRUCTURE OF CYTOCHROME P450ERYF WITH ANDROSTENDIONE BOUND

PDB ID 1eup

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