1ez4

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[[Image:1ez4.gif|left|200px]]<br /><applet load="1ez4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ez4, resolution 2.3&Aring;" />
 
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'''CRYSTAL STRUCTURE OF NON-ALLOSTERIC L-LACTATE DEHYDROGENASE FROM LACTOBACILLUS PENTOSUS AT 2.3 ANGSTROM RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF NON-ALLOSTERIC L-LACTATE DEHYDROGENASE FROM LACTOBACILLUS PENTOSUS AT 2.3 ANGSTROM RESOLUTION==
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L-Lactate dehydrogenase (LDH) from Lactobacillus pentosus is a non-allosteric enzyme, which shows, however, high sequence similarity to allosteric LDHs from certain bacteria. To elucidate the structural basis of the absence of allostery of L. pentosus LDH (LPLDH), we determined the crystal structure of LPLDH at 2.3 A resolution. Bacterial LDHs are tetrameric enzymes composed of identical subunits and exhibit 222 symmetry. The quaternary structure of LPLDH was similar to the active conformation of allosteric LDHs. Structural analysis revealed that the subunit interfaces of LPLDH are optimized mainly through hydrophilic interactions rather than hydrophobic interactions, compared with other LDHs. The subunit interfaces of LPLDH are more specifically stabilized by increased numbers of intersubunit salt bridges and hydrogen bonds, and higher geometrical complementarity. Such high specificity at the subunit interfaces should hinder the rearrangement of the quaternary structure needed for allosteric regulation and thus explain the "non-allostery" of LPLDH.
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<StructureSection load='1ez4' size='340' side='right'caption='[[1ez4]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ez4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_pentosus Lactiplantibacillus pentosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EZ4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ez4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ez4 OCA], [https://pdbe.org/1ez4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ez4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ez4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ez4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LDH_LACPE LDH_LACPE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/1ez4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ez4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EZ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_pentosus Lactobacillus pentosus] with <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/L-lactate_dehydrogenase L-lactate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.27 1.1.1.27] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZ4 OCA].
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*[[Lactate dehydrogenase 3D structures|Lactate dehydrogenase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of non-allosteric L-lactate dehydrogenase from Lactobacillus pentosus at 2.3 A resolution: specific interactions at subunit interfaces., Uchikoba H, Fushinobu S, Wakagi T, Konno M, Taguchi H, Matsuzawa H, Proteins. 2002 Feb 1;46(2):206-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11807949 11807949]
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[[Category: Lactiplantibacillus pentosus]]
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[[Category: L-lactate dehydrogenase]]
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[[Category: Large Structures]]
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[[Category: Lactobacillus pentosus]]
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[[Category: Fushinobu S]]
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[[Category: Single protein]]
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[[Category: Konno M]]
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[[Category: Fushinobu, S.]]
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[[Category: Matsuzawa H]]
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[[Category: Konno, M.]]
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[[Category: Taguchi H]]
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[[Category: Matsuzawa, H.]]
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[[Category: Uchikoba H]]
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[[Category: Taguchi, H.]]
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[[Category: Wakagi T]]
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[[Category: Uchikoba, H.]]
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[[Category: Wakagi, T.]]
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[[Category: NAD]]
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[[Category: rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:33:14 2008''
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Current revision

CRYSTAL STRUCTURE OF NON-ALLOSTERIC L-LACTATE DEHYDROGENASE FROM LACTOBACILLUS PENTOSUS AT 2.3 ANGSTROM RESOLUTION

PDB ID 1ez4

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