1f3u

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(New page: 200px<br /> <applet load="1f3u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f3u, resolution 1.70&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1f3u.gif|left|200px]]<br />
 
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<applet load="1f3u" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f3u, resolution 1.70&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE RAP30/74 INTERACTION DOMAINS OF HUMAN TFIIF'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE RAP30/74 INTERACTION DOMAINS OF HUMAN TFIIF==
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General transcription factor IIF (TFIIF) is required for transcription by, RNA polymerase II; it consists minimally of a heterodimer of RNA, polymerase-associated proteins RAP30 and RAP74. According to solution and, mutagenesis studies, the multiple domains of RAP30 and RAP74 bind PolII, TFIIB, TAF250 and DNA in interactions that are essential for transcription, initiation and elongation. The X-ray structure of the RAP30/RAP74, interaction domains at 1.7 A resolution reveals a novel "triple barrel", dimerization fold and suggests with mutant data that interactions with the, transcription apparatus are mediated not only by this tripartite, beta-barrel, but also via flexible loops and alpha and beta-structures, extending from it.
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<StructureSection load='1f3u' size='340' side='right'caption='[[1f3u]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f3u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F3U FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f3u OCA], [https://pdbe.org/1f3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f3u RCSB], [https://www.ebi.ac.uk/pdbsum/1f3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f3u ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/T2FB_HUMAN T2FB_HUMAN] TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.<ref>PMID:2477704</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/1f3u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f3u ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1F3U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F3U OCA].
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*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
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== References ==
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==Reference==
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<references/>
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Novel dimerization fold of RAP30/RAP74 in human TFIIF at 1.7 A resolution., Gaiser F, Tan S, Richmond TJ, J Mol Biol. 2000 Oct 6;302(5):1119-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11183778 11183778]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Gaiser, F.]]
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[[Category: Gaiser F]]
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[[Category: Richmond, T.J.]]
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[[Category: Richmond TJ]]
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[[Category: Tan, S.]]
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[[Category: Tan S]]
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[[Category: beta barrel]]
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[[Category: general transcription initiation and elongation factor]]
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[[Category: novel dimerization fold]]
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[[Category: rna polymerase ii]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:48:48 2007''
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Current revision

CRYSTAL STRUCTURE OF THE RAP30/74 INTERACTION DOMAINS OF HUMAN TFIIF

PDB ID 1f3u

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