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1f5v

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(New page: 200px<br /><applet load="1f5v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f5v, resolution 1.7&Aring;" /> '''STRUCTURE AND SITE-DI...)
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[[Image:1f5v.jpg|left|200px]]<br /><applet load="1f5v" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f5v, resolution 1.7&Aring;" />
 
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'''STRUCTURE AND SITE-DIRECTED MUTAGENESIS OF A FLAVOPROTEIN FROM ESCHERICHIA COLI THAT REDUCES NITROCOMPOUNDS. ALTERATION OF PYRIDINE NUCLEOTIDE BINDING BY A SINGLE AMINO ACID SUBSTITUTION'''<br />
 
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==Overview==
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==STRUCTURE AND SITE-DIRECTED MUTAGENESIS OF A FLAVOPROTEIN FROM ESCHERICHIA COLI THAT REDUCES NITROCOMPOUNDS. ALTERATION OF PYRIDINE NUCLEOTIDE BINDING BY A SINGLE AMINO ACID SUBSTITUTION==
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The crystal structure of a major oxygen-insensitive nitroreductase (NfsA), from Escherichia coli has been solved by the molecular replacement method, at 1.7-A resolution. This enzyme is a homodimeric flavoprotein with one, FMN cofactor per monomer and catalyzes reduction of nitrocompounds using, NADPH. The structure exhibits an alpha + beta-fold, and is comprised of a, central domain and an excursion domain. The overall structure of NfsA is, similar to the NADPH-dependent flavin reductase of Vibrio harveyi, despite, definite difference in the spatial arrangement of residues around the, putative substrate-binding site. On the basis of the crystal structure of, NfsA and its alignment with the V. harveyi flavin reductase and the, NADPH-dependent nitro/flavin reductase of Bacillus subtilis, residues, Arg(203) and Arg(208) of the loop region between helices I and J in the, vicinity of the catalytic center FMN is predicted as a determinant for, NADPH binding. The R203A mutant results in a 33-fold increase in the K(m), value for NADPH indicating that the side chain of Arg(203) plays a key, role in binding NADPH possibly to interact with the 2'-phosphate group.
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<StructureSection load='1f5v' size='340' side='right'caption='[[1f5v]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F5V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5v OCA], [https://pdbe.org/1f5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f5v RCSB], [https://www.ebi.ac.uk/pdbsum/1f5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f5v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NFSA_ECOLI NFSA_ECOLI] Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen-insensitive nitroreductase activity in E.coli.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/1f5v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f5v ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1F5V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/NADPH_dehydrogenase_(quinone) NADPH dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.6 1.6.99.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F5V OCA].
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*[[Nitroreductase 3D structures|Nitroreductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure and site-directed mutagenesis of a flavoprotein from Escherichia coli that reduces nitrocompounds: alteration of pyridine nucleotide binding by a single amino acid substitution., Kobori T, Sasaki H, Lee WC, Zenno S, Saigo K, Murphy ME, Tanokura M, J Biol Chem. 2001 Jan 26;276(4):2816-23. Epub 2000 Oct 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11034992 11034992]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: NADPH dehydrogenase (quinone)]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Kobori T]]
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[[Category: Kobori, T.]]
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[[Category: Lee WC]]
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[[Category: Lee, W.C.]]
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[[Category: Murphy MEP]]
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[[Category: Murphy, M.E.P.]]
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[[Category: Saigo K]]
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[[Category: Saigo, K.]]
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[[Category: Sasaki H]]
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[[Category: Sasaki, H.]]
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[[Category: Tanokura M]]
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[[Category: Tanokura, M.]]
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[[Category: Zenno S]]
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[[Category: Zenno, S.]]
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[[Category: FMN]]
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[[Category: escherichia coli]]
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[[Category: flavoprotein]]
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[[Category: nitrocompound]]
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[[Category: nitroreductase]]
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[[Category: oxidoreduction]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:38:32 2007''
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Current revision

STRUCTURE AND SITE-DIRECTED MUTAGENESIS OF A FLAVOPROTEIN FROM ESCHERICHIA COLI THAT REDUCES NITROCOMPOUNDS. ALTERATION OF PYRIDINE NUCLEOTIDE BINDING BY A SINGLE AMINO ACID SUBSTITUTION

PDB ID 1f5v

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