1feh

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[[Image:1feh.gif|left|200px]]<br /><applet load="1feh" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1feh, resolution 1.8&Aring;" />
 
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'''FE-ONLY HYDROGENASE FROM CLOSTRIDIUM PASTEURIANUM'''<br />
 
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==Overview==
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==FE-ONLY HYDROGENASE FROM CLOSTRIDIUM PASTEURIANUM==
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A three-dimensional structure for the monomeric iron-containing hydrogenase (CpI) from Clostridium pasteurianum was determined to 1.8 angstrom resolution by x-ray crystallography using multiwavelength anomalous dispersion (MAD) phasing. CpI, an enzyme that catalyzes the two-electron reduction of two protons to yield dihydrogen, was found to contain 20 gram atoms of iron per mole of protein, arranged into five distinct [Fe-S] clusters. The probable active-site cluster, previously termed the H-cluster, was found to be an unexpected arrangement of six iron atoms existing as a [4Fe-4S] cubane subcluster covalently bridged by a cysteinate thiol to a [2Fe] subcluster. The iron atoms of the [2Fe] subcluster both exist with an octahedral coordination geometry and are bridged to each other by three non-protein atoms, assigned as two sulfide atoms and one carbonyl or cyanide molecule. This structure provides insights into the mechanism of biological hydrogen activation and has broader implications for [Fe-S] cluster structure and function in biological systems.
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<StructureSection load='1feh' size='340' side='right'caption='[[1feh]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1feh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. The March 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Hydrogenase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_3 10.2210/rcsb_pdb/mom_2009_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FEH FirstGlance]. <br>
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1FEH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum] with <scene name='pdbligand=HC1:'>HC1</scene>, <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FEH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HC1:2+IRON/2+SULFUR/5+CARBONYL/2+WATER+INORGANIC+CLUSTER'>HC1</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1feh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1feh OCA], [https://pdbe.org/1feh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1feh RCSB], [https://www.ebi.ac.uk/pdbsum/1feh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1feh ProSAT]</span></td></tr>
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X-ray crystal structure of the Fe-only hydrogenase (CpI) from Clostridium pasteurianum to 1.8 angstrom resolution., Peters JW, Lanzilotta WN, Lemon BJ, Seefeldt LC, Science. 1998 Dec 4;282(5395):1853-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9836629 9836629]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHF1_CLOPA PHF1_CLOPA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/1feh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1feh ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Clostridium pasteurianum]]
[[Category: Clostridium pasteurianum]]
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[[Category: Ferredoxin hydrogenase]]
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[[Category: Hydrogenase]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Lanzilotta, W N.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Lemon, B J.]]
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[[Category: Lanzilotta WN]]
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[[Category: Peters, J W.]]
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[[Category: Lemon BJ]]
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[[Category: Seefeldt, L C.]]
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[[Category: Peters JW]]
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[[Category: FES]]
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[[Category: Seefeldt LC]]
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[[Category: HC1]]
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[[Category: SF4]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:48 2008''
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Current revision

FE-ONLY HYDROGENASE FROM CLOSTRIDIUM PASTEURIANUM

PDB ID 1feh

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