1fhy

<StructureSection load='1fhy' size='340' side='right' caption='[[1fhy]], [[Resolution|resolution]] 2.20&Aring;' scene=''>

<table><tr><td colspan='2'>[[1fhy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FHY FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PSO:4-HYDROXYMETHYL-4,5,8-TRIMETHYLPSORALEN'>PSO</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhy OCA], [http://pdbe.org/1fhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fhy RCSB], [http://www.ebi.ac.uk/pdbsum/1fhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fhy ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.

The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions.,Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:11302703<ref>PMID:11302703</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1fhy" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Alberti, M]]

[[Category: Eichman, B F]]

[[Category: Mooers, B H.M]]

[[Category: 4-way junction]]

[[Category: Cross-linked dna]]

[[Category: Dna]]

[[Category: Dna-drug complex]]

[[Category: Psoralen]]