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1fm8

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CHALCONE ISOMERASE COMPLEXED WITH 5,4'-DIDEOXYFLAVANONE

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Retrieved from "http://52.214.119.220/wiki/index.php/1fm8"

Categories: Large Structures | Medicago sativa | Jez JM | Noel JP

@@ Line 1: / Line 1: @@
-[[Image:1fm8.jpg|left|200px]]
- {{Structure
+==CHALCONE ISOMERASE COMPLEXED WITH 5,4'-DIDEOXYFLAVANONE==
-|PDB= 1fm8 |SIZE=350|CAPTION= <scene name='initialview01'>1fm8</scene>, resolution 2.30&Aring;
+<StructureSection load='1fm8' size='340' side='right'caption='[[1fm8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=DDC:7-HYDROXY-2-PHENYL-CHROMAN-4-ONE'>DDC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+<table><tr><td colspan='2'>[[1fm8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FM8 FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chalcone_isomerase Chalcone isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.6 5.5.1.6] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDC:7-HYDROXY-2-PHENYL-CHROMAN-4-ONE'>DDC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fm8 OCA], [https://pdbe.org/1fm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fm8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fm8 ProSAT]</span></td></tr>
-|RELATEDENTRY=[[1eyp|1EYP]], [[1eyq|1EYQ]], [[1fm7|1FM7]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fm8 OCA], [http://www.ebi.ac.uk/pdbsum/1fm8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fm8 RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/CFI1_MEDSA CFI1_MEDSA] Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin.<ref>PMID:10966651</ref> <ref>PMID:11955065</ref> <ref>PMID:11698411</ref>
+== Evolutionary Conservation ==
-'''CHALCONE ISOMERASE COMPLEXED WITH 5,4'-DIDEOXYFLAVANONE'''
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
-==Overview==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/1fm8_consurf.spt"</scriptWhenChecked>
-Chalcone isomerase (CHI) catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. The activity of CHI is essential for the biosynthesis of flavanone precursors of floral pigments and phenylpropanoid plant defense compounds. We have examined the spontaneous and CHI-catalyzed cyclization reactions of 4,2',4',6'-tetrahydroxychalcone, 4,2',4'-trihydroxychalcone, 2',4'-dihydroxychalcone, and 4,2'-dihydroxychalcone into the corresponding flavanones. The pH dependence of flavanone formation indicates that both the non-enzymatic and enzymatic reactions first require the bulk phase ionization of the substrate 2'-hydroxyl group and subsequently on the reactivity of the newly formed 2'-oxyanion during C-ring formation. Solvent viscosity experiments demonstrate that at pH 7.5 the CHI-catalyzed cyclization reactions of 4,2',4',6'-tetrahydroxychalcone, 4,2',4'-trihydroxychalcone, and 2',4'-dihydroxychalcone are approximately 90% diffusion-controlled, whereas cyclization of 4,2'-dihydroxychalcone is limited by a chemical step that likely reflects the higher pK(a) of the 2'-hydroxyl group. At pH 6.0, the reactions with 4,2',4',6'-tetrahydroxychalcone and 4,2',4'-trihydroxychalcone are approximately 50% diffusion-limited, whereas the reactions of both dihydroxychalcones are limited by chemical steps. Comparisons of the 2.1-2.3 A resolution crystal structures of CHI complexed with the products 7,4'-dihydroxyflavanone, 7-hydroxyflavanone, and 4'-hydroxyflavanone show that the 7-hydroxyflavanones all share a common binding mode, whereas 4'-hydroxyflavanone binds in an altered orientation at the active site. Our functional and structural studies support the proposal that CHI accelerates the stereochemically defined intramolecular cyclization of chalcones into biologically active (2S)-flavanones by selectively binding an ionized chalcone in a conformation conducive to ring closure in a diffusion-controlled reaction.
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==About this Structure==
+  </jmolCheckbox>
-FM8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FM8 OCA].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fm8 ConSurf].
+<div style="clear:both"></div>
-==Reference==
+== References ==
-Reaction mechanism of chalcone isomerase. pH dependence, diffusion control, and product binding differences., Jez JM, Noel JP, J Biol Chem. 2002 Jan 11;277(2):1361-9. Epub 2001 Nov 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11698411 11698411]
+<references/>
-[[Category: Chalcone isomerase]]
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Medicago sativa]]
-[[Category: Single protein]]
+[[Category: Jez JM]]
-[[Category: Jez, J M.]]
+[[Category: Noel JP]]
-[[Category: Noel, J P.]]
-[[Category: chalcone]]
-[[Category: diffusion-limited]]
-[[Category: natural product biosynthesis]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:25:44 2008''

1fm8

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CHALCONE ISOMERASE COMPLEXED WITH 5,4'-DIDEOXYFLAVANONE

Structural highlights

Function

Evolutionary Conservation

References

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